牛奶中脂肪酸相关性及其样本容量的确定

为分析牛奶中各种脂肪酸的变异性和相关性,确定采样所需适宜的样本容量,以大兴区泌乳早期奶牛为研究对象,利用气相色谱对牛奶中的脂肪酸进行测定,数据通过SAS 8.0进行统计分析。结果显示:牛奶中脂肪酸的变异范围为5.57%～28.02%;C6～C14脂肪酸之间呈极显著的正相关,C17、C18、c9C18:1与C6～C16脂肪酸有显著的负相关关系;在95%置信度条件下,采样适宜的样本容量为10～16。牛奶脂肪酸之间存在广泛的关联性,试验设计时,可以根据研究脂肪酸的不同确定所需的样本容量。

Correlation of the fatty acids in milk and determination of the study sample size

To determine correlation and variation of milk fatty acids and figure out proper sample size in experiments,dairy cattle in early lactation from Daxing farm were used in this study.The determination for fatty acids in milk was accomplished through gas chromatography and data were analyzed by SAS 8.0.The results suggested that coefficients of variation of FA in milk varied from 5.57% to 28.02%.There are significant positive correlations among C6-C14,however,negative correlations were observed between C17,C18,c9C18:1 and C6-C16.The best sample size for experiments would be 10~16 with the confidence level of 95%.There were extensive correlation existed among FA in milk,and the FA sample size for studies should be determined especially according to the targets.