利用AFLP标记构建家蚕分子连锁图谱

 ＡＦＬＰ技术构建了家蚕分子标记连锁图谱,在扩增中使用了１８组双引物及两个单引物在家蚕品系７８２和ｏｄ １００的回交子代中得到了１１７７个扩增位点,有３５９个非分离位点,８１８个分离位点。分离位点中有２９２个位点符合孟德尔１：１分离比例。用ＭＡＰＭＡＫＥＲ软件将２９２个标记位点及形态标记基因ｏｄ分成２６个连锁群,１９个二联体及８１个未进入连锁关系的标记。每个连锁群包含３～１４个标记。平均为 ６．５个标记。该连锁图总图距为 ３２８５．５ｃＭ。标记间平均距离为 ２０．０ｃＭ。形态标记ｏｄ被定位于该图谱中 ３号连锁群上,而ｏｄ基因位于家蚕Ｚ染色体上,故可确定该分子标记连锁群对应于传统的家蚕形态标记图谱中的Ｚ染色体。

Construction of Molecular Map of the Silkworm (Bombyx mori. L. ) Using AFLP Markers

An AFLP linkage map of Bomb yr mon . L. was constructed using Pst I and Taq I to digest genome DNA and 18 primer combinations and 2 single primers. 1177 clear polymorphic bands in the BC1 population of No. 782 and od 100 cross were detected. of which 292 bands fell the expected segregation ratio of 1: 1 at P=0. 05 level. The "GROUP" command (LOD 2. 5, maximum recombination value 0. 3) was used to segregate these 292 informative markers into 26 linkage groups. The "COMPARE", "TRY", "ORDER", "RIPPLE" commands were used to map markers per group typing error was detected using ERROR DETECTION option. Map distance between neighboring loci was calculated using the Kosambi mapping function. The AFLP map consists of 169 loci and the od gene, and the total length of the map is 3285. 5 cM. Linkage group 3 on our map corresponds to chromosome Z on the conventional linkage map due to the od gene.