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苦菜的组织培养与快速繁殖
引用本文:邱奉同,张渝洁,张利.苦菜的组织培养与快速繁殖[J].安徽农业科学,2005,33(2):241-242.
作者姓名:邱奉同  张渝洁  张利
作者单位:临沂师范学院生,命科学学院,山东临沂,276005;临沂师范学院生,命科学学院,山东临沂,276005;临沂师范学院生,命科学学院,山东临沂,276005
摘    要:通过对苦菜的组织培养 ,研究了外植体不同取材部位及不同激素种类、浓度对愈伤组织及芽诱导和增殖的影响。结果表明 :苦菜根的繁殖能力很强 ,但分化时间稍长 ;2 ,4 -D对愈伤组织的诱导能力较强 ,NAA对芽的诱导能力较强。同时 ,试验还筛选出了诱导愈伤组织的适宜培养基 :MS + 6 BA 2 .0mg/L + 2 ,4 -D 0 .2mg/L ;芽分化培养基 :MS + 6 BA 2 .0mg/L +NAA 0 .0 5mg/L ;继代培养基 :MS + 6 BA2 .0mg/L +NAA 0 .2mg/L。

关 键 词:苦菜  组织培养  快速繁殖
文章编号:0517-6611(2005)02-0241-02

Tissue Culture and Rapid Propagation of Ixeris Chinensis
QIU Feng-tong et al.Tissue Culture and Rapid Propagation of Ixeris Chinensis[J].Journal of Anhui Agricultural Sciences,2005,33(2):241-242.
Authors:QIU Feng-tong
Abstract:The influence of different parts, different hormones and their concentrations on Ixeris Chinensi tissue culture inducing callus formation and bud growth was studied. The results showed that: during Ixeris Chinensis tissue culture, the root was easy to be induced, but it took more time to form bud. Through studying different hormones inducing buds and callus of Ixeris Chinensis,we drew that: 2,4-D induced callus more easily and NAA induced buds more easily. Simultaneously, in the experiment the appropriate media for the callus inducing was MS+6-BA2.0mg/L+2,4-D 0.2mg/L,buds differentiation and subculture had been picked:MS+6-BA2.0mg/L+NAA0.05mg/L,MS+6-BA2.0mg/L+NAA0.2mg/L.
Keywords:Ixeris Chinensis  Tissue culture  Rapid propagation
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