| 辣椒花药培养中若干影响因素的研究 |
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| 引用本文： | 王立浩,张宝玺,郭家珍,杨桂梅,堵玫珍.辣椒花药培养中若干影响因素的研究[J].园艺学报,2004,31(2):199-204. |
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| 作者姓名： | 王立浩 张宝玺 郭家珍 杨桂梅 堵玫珍 |
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| 作者单位： | (中国农业科学院蔬菜花卉研究所，北京100081) |
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| 基金项目： | 国家“863”重大专项资助项目(2002AA207012-1) |
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| 摘 要： | 以4个辣椒品种(组合)为试材，通过正交试验，按不同取蕾时期，在培养基中添加不同浓度的硝酸银，维生素C、植物生长调节剂、氮源、碳源、核酸、活性炭等研究对辣椒花药培养中组织褐化、愈伤组织发生率、胚状体的发生率的影响。结果表明：硝酸银、维生素c能有效地减轻组织褐化；对于辣椒花药培养愈伤组织生成率影响较大的前4位依次是植物生长调节剂、活性炭、硝酸银、维生素C；对于胚状体发生率影响较大的前4位依次是维生素C、取蕾时期、碳源、硝酸银。
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| 关 键 词： | 辣椒 花药培养 愈伤组织 胚状体发生 褐化 培养基 |
| 文章编号： | 0513-353X(2004)02-0199-06 |
| 收稿时间： | 2003-10-9 |
| 修稿时间： | 2004-2-24 |
Studies of Effects of Several Factors on Anther Culture of Capsicum annuum L. |
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| Wang Lihao,Zhang Baoxi,Guo Jiazhen,Yang Guimei,and Du Meizhen.Studies of Effects of Several Factors on Anther Culture of Capsicum annuum L.[J].Acta Horticulturae Sinica,2004,31(2):199-204. |
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| Authors: | Wang Lihao Zhang Baoxi Guo Jiazhen Yang Guimei and Du Meizhen |
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| Institution: | (Institute of Vegetables and Flowers of Chinese Academy of Agricultural Sciences，Beijing 100081，China) |
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| Abstract: | Four genotypes of pepper were used to study the effects of AgNO3, Vitamin C , carbon source, nitrogen source, harvest time, plant growth regulator on the anther culture of Capsicum annuum L. through orthogonal tests. As the results, AgNO3, Vitamin C can reduce the browning of the tissues; Plant growth regulator, active carbon, AgNO3, Vitamin C have a higher effect on calli yields; Vitamin C, harvest time, car-born source, AgNO3 have a higher effect on embryogenesis. A new modified culture medium was concluded. |
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| Keywords: | Capsicum annuum L Anther culture Embryogenesis Callus Browning Medium |
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