| 无刺红树莓组织培养快速繁殖技术研究 |
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| 引用本文： | 朴日子,曹后男,陈艳秋,宗成文,陈蕾.无刺红树莓组织培养快速繁殖技术研究[J].吉林农业大学学报,2006,28(4):411-414. |
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| 作者姓名： | 朴日子 曹后男 陈艳秋 宗成文 陈蕾 |
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| 作者单位： | 延边大学农学院,龙井,133400 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 以无刺红树莓带芽茎段和茎尖为外植体,筛选各组织培养阶段高频、稳定的适宜培养基。结果表明:外植体以茎段为佳。分化培养基以1/2 MS 6-BA 1.0 mg/L 活性炭0.1%最好,分化率可达100%;继代增殖培养基以MS 6-BA 1.0 mg/L NAA 0.1 mg/L最理想,增殖倍数可达7倍;生根培养基以1/2 MS IBA1.0 mg/L 活性炭1.0 g/L最佳,生根率可达93.33%。生根类型多为皮部生根。采用2步移栽法进行移裁时,第1次移裁以河砂为基质移栽成活率达到98%,第2次移裁成活率100%。
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| 关 键 词： | 无刺红树莓 茎段 组织培养 激素 |
| 文章编号： | 1000-5684（2006）04-0411-04 |
| 收稿时间： | 2005-12-24 |
| 修稿时间： | 2005-12-242006-01-16 |
Study on Tissue Culture and Rapid Propagation of Thornless Red Raspberry Idaeus |
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| PIAO Ri-zi,CAO Hou-nan,CHEN Yan-qiu,ZONG Cheng-wen,CHEN Lei.Study on Tissue Culture and Rapid Propagation of Thornless Red Raspberry Idaeus[J].Journal of Jilin Agricultural University,2006,28(4):411-414. |
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| Authors: | PIAO Ri-zi CAO Hou-nan CHEN Yan-qiu ZONG Cheng-wen CHEN Lei |
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| Institution: | College of Agriculture, Yanbian University, Longiing 133400, China |
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| Abstract: | Using stem segments with buds and tips as explants,tube rapid propagation of Red Raspberry was studied.The results indicated that the appropriate media for respective culture stages were: for germination of auxiliary buds: 1/2 MS 6-BA(1.0 mg/L);for differentiation and multiplication: MS 6-BA 1.0 mg/L NAA 0.1 mg/L,the multiple for multiplication was up to 7;for rooting: 1/2MS IBA(1.0 mg/L )active carbon 1.0 g/L and the rate of rooting was 93.33%.After 20-day acclimatization,the rooted plantlets were transplanted to substrata in two successive steps,the survival rates of which were 98% and 100% respectively. |
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| Keywords: | thornless red raspberry stem tissue culture hormone |
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