新疆维药阿里红液体发酵培养基优化研究

浸粉0.3;、蛋白胨0.75;、酵母浸粉0.5;.经12 d发酵,每50 mL发酵液菌体干重和胞外粗多糖含量最高可达65.4和77.7 mg.

Optimization of Liquid Fermentation Medium by Fomes officinalis Ames of Uygur Traditional Medicine

Extracellular polysaccharide of Fomes Officinalis Ames were used widely with the functions of immune- enhancing and anti - senescence.This study was conducted to optimize the nutrient component, mixture ratio and concentration,growth factor and best combination of all elements through single factor and orthogonal test to produce extracellular polysaccharide by the wild strain of Fomes offcinalis Ames of Uygur Traditional Medicine.The optimal content of seed and fermentation media were obtained according to the yield of biomass and extracellular polysaccharide were as follows,seed medium:glucose 3%,peptone 1.5%, KH_2PO_4 0.3%,MgSO_4·7H_2O 0.1%,VB_1 0.1 mg/mL;fermentation medium:glucose 1%,malt extract 0.3%, peptone 0.75%.The results showed that with the optimized seed and fermentation media,the content of dry weight and extracellular polysaccharide reach 65.4 mg and 77.7 mg per 50 mL broth content,respectively when it was fermented for 12 d.