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火炬姜离体快繁技术研究
引用本文:潘学峰,王昌茂.火炬姜离体快繁技术研究[J].园艺学报,2003,30(2):183-186.
作者姓名:潘学峰  王昌茂
作者单位:(海南大学农学院,海口 570228)
摘    要: 试验表明:火炬姜组培快繁以Ms+6-BA 2.0mg/L+NAA 0.1 mg/L+CW 10%作为增殖培养基可以获得理想的效果,增殖倍数可达4.3倍;以双芽为外植体在该培养基上经30 d培养可增殖5.4倍;在不含任何激素的1/2 Ms+0.15%活性炭培养基上即可形成较发达的根系,生根率达100%;试管苗移栽对基质要求不严,移栽到疏松透气、富含有机质的表土中,成活率高达100%,而且植株生长良好。

关 键 词:火炬姜  丛生芽  组培快繁
文章编号:0513-353X(2003)02-0183-04
收稿时间:2002-6-5
修稿时间:2002年6月5日

Studies on Rapid in Vitro Propagation of Phaeomeria magnifica
Pan Xuefeng and Wang Changmao.Studies on Rapid in Vitro Propagation of Phaeomeria magnifica[J].Acta Horticulturae Sinica,2003,30(2):183-186.
Authors:Pan Xuefeng and Wang Changmao
Institution:(Agriculturae College,Hainan,Haikou 570228,China)
Abstract:The research result of the in vitro propagation of the Phaeomeria magnifica is presented in this paper.The results showed that:Using MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+CW 10% as proliferation medium,an optimal proliferation rate was obtained,the proliferation rate could reach 4.3 times;Using double shoots as explants,after 30 days of culture in this medium,it could amplify 5.4 times.The root formation of Phaeomeria magnifica tissue culture shoots had a low demand for exotic hormones,even in a medium of no hormones it could still form a good root system,the rooting rate could reach 100%.The transplantation of Phaeomeria magnifica tissue culture shoots was simple and feasible.There was no strict demand for matrix,when the plantlets were transplanted to superficial soil,the survival rate could reach 100%,and the plants grew well.
Keywords:Phaeomeria magnifica  Adventitious shoot  Rapid propagation via tissue culture
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