| Research on Tobacco Transformation of Vacuolar H＋-ATPase Subunit c Gene from Iris lacteal |
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| 作者姓名： | Wu Duo Zhou Ai-min Shi Sheng-feng Li Shan-shan Wang Jin-gang |
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| 基金项目： | Supported by the Post-doctoral Research Start-up Funding of Heilongjiang Province （LBH-210144）; the Post-doctoral Funds Project of Heilongjiang Province （520-415037） |
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| 摘 要： | Vacuolar ATPase is the key enzyme in the aspect of plant stress, which contains 13 subunits. In this study, VHA-c which was cloned from the salt-tolerant plants Iris lacteal was transformed into tobacc...
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| 收稿时间： | 11 March 2011 |
Research on Tobacco Transformation of Vacuolar H+-ATPase Subunit c Gene from Iris lacteal |
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| Wu Duo,Zhou Ai-min,Shi Sheng-feng,Li Shan-shan,Wang Jin-gang.Research on Tobacco Transformation of Vacuolar H+-ATPase Subunit c Gene from Iris lacteal[J].Journal of Northeast Agricultural University,2012,19(3):43-48. |
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| Authors: | Wu Duo Zhou Ai-min Shi Sheng-feng Li Shan-shan Wang Jin-gang |
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| Institution: | College of Horticulture, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | Vacuolar ATPase is the key enzyme in the aspect of plant stress, which contains 13 subunits. In this study, VHA-c which was cloned from the salt-tolerant plants Iris lacteal was transformed into tobacco, and then treated the transgenic tobacco with 0.7% NaCl for 7 days. Detected the MDA content, the SOD activity and the POD activity. Moreover, the transgenic plants displayed obviously better growth than the normal plants under salt stress. These results suggested that VHA-c could confer the stress tolerance through enhancing the POD and the SOD activities, and by protecting membranes from damage by decreasing lipid peroxidation under salt stress. |
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| Keywords: | VHA-c Nicotiana tabacum L cv salt stress |
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