| 链霉素人工抗原的制备与鉴定 |
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| 引用本文： | 范国英,王顺岗,王自良,邓润广,张改平,杨艳艳. 链霉素人工抗原的制备与鉴定[J]. 西北农业学报, 2009, 18(2): 45-50 |
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| 作者姓名： | 范国英 王顺岗 王自良 邓润广 张改平 杨艳艳 |
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| 作者单位： | 1. 河南科技学院,动物科学学院,河南新乡,453003
2. 河南省农业科学院,免疫学重点实验室,河南郑州,450002 |
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| 摘 要： | 链霉素经氧-羧甲基羟胺肟化处理后引入羧基,采用EDC一步法分别合成免疫原BSA-EDC-SM和包被抗原OVA-EDC-SM;用戊二醛法和1,4-丁二醇缩水甘油醚分别合成BSA-GA-SM和BSA-di-SM;采用SDS-PAGE凝胶电泳和紫外扫描测得BSA-EDC-SM、BSA-GA-SM和BSA-di-SM的BSA与SM的分子结合比分别为1:23、1:15和1:10.用3种免疫原免疫Balb/C小鼠,获得了高价、敏感和特异的抗血清.BSA-EDC-SM免疫组中1#小鼠的多克隆抗体对SM的IC50为45.8üg/mL,对双氢链霉素(DHSM)的交叉反应为90.6%,与其他氨基糖苷类药物或抗生素药物的交叉反应均小于0.001%.BSA-di-SM和BSA-GA-SM免疫组的多克隆抗体对DHSM、其他氨基糖苷类药物或抗生素类药物的交叉反应均小于0.001%.
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| 关 键 词： | 链霉素 人工抗原 制备 鉴定 |
| 收稿时间： | 2008-09-10 |
| 修稿时间： | 2008-12-01 |
Preparation and Idenfication of Artificial Antigen of Streptomycin |
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| FAN Guoying,WANG Shungang,WANG Ziliang,DENG Runguang,ZHANG Gaiping and YANG Yanyan. Preparation and Idenfication of Artificial Antigen of Streptomycin[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2009, 18(2): 45-50 |
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| Authors: | FAN Guoying WANG Shungang WANG Ziliang DENG Runguang ZHANG Gaiping YANG Yanyan |
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| Affiliation: | The Animal Science College, Henan Institute of Science and Technology, Xinxiang He''nan 453003, China;The Animal Science College, Henan Institute of Science and Technology, Xinxiang He''nan 453003, China;The Animal Science College, Henan Institute of Science and Technology, Xinxiang He''nan 453003, China;Institute of Biology and Technique Science, Agricultural Academy of Henan Province, Zhengzhou He''nan 450002, China;Institute of Biology and Technique Science, Agricultural Academy of Henan Province, Zhengzhou He''nan 450002, China;Institute of Biology and Technique Science, Agricultural Academy of Henan Province, Zhengzhou He''nan 450002, China |
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| Abstract: | |
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| Keywords: | Streptomycin Artificial antigen Identification |
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