Canine specific ELISA for coagulation factor VII |
| |
| Authors: | Knudsen Tom Kjelgaard-Hansen Mads Tranholm Mikael Wiinberg Bo Clausen Jes T Hansen Jens Jacob Nichols Timothy C Kjalke Marianne Jensen Asger L Kristensen Annemarie T |
| |
| Affiliation: | aDepartment of Small Animal Clinical Sciences, Faculty of Life Sciences, University of Copenhagen, Copenhagen DK-1870, Denmark;bNovo Nordisk A/S, Maaloev DK-2760, Denmark;cDepartment of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27516, USA;dDepartment of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C DK-1870, Denmark |
| |
| Abstract: | Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable in the diagnosis of hereditary canine FVII deficiency. |
| |
| Keywords: | FVII deficiency Haemostasis Coagulation factor ELISA Plasma Immunoassays |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
