钙处理对乙烯诱导的番茄离体花柄脱落的抑制作用

 以番茄‘辽园多丽’的小花为材料, 研究外源乙烯( 20μL ·L^-1 ) 处理下, 花柄脱落过程中, 离区微粒体膜Ca²⁺ -ATPase、羧甲基纤维素钠酶(CMC-Na) 、内切多聚半乳糖醛酸酶( Endo-PG) 和果胶脂酶( PE) 等活性的变化。结果表明: 外源乙烯加速了花柄的脱落, 刺激了其离区的Ca²⁺ -ATPase活性先升高后下降, 随后离区CMC-Na、Endo-PG、PE活性升高; 钙离子专一性螯合剂EGTA和钙调素拮抗剂TFP均在一定程度上抑制了乙烯诱导的上述反应, 表明细胞内Ca²⁺与CaM参与了乙烯反应。

Physiological Regulation of Calcium in Tomato Pedicel in Vitro During Abscission Induced by Ethylene

In an experiment, pedicels of Lycopersicon esculenturm Mill. ‘Liaoyuanduoli’were used to study the changes of Ca²⁺ -ATPase activity, abscission rate and the activity of Endo-PG, PE, CMC-Na enzymes in abscission zone after the pedicelswere incubated in exogenous ethylene (20μL·L^-1 ). The resultsshowed that ethylene could accelerate the abscission during incubation, Ca²⁺ -ATPase activity first rose sharply and then declined quickly. Subsequently, there was a increase in the activity of Endo-PG, PE, CMC-Na enzyme in abscission zone. Calmodulin antagonist trifluoperizine (TFP) and Ca²⁺ chelator EGTA both inhibited the effects induced by ethylene to a certain extent. It is concluded that Ca²⁺ and CaM participate in the reaction of exogenous ethylene.