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蓝狐GDF-8多态性及其血液生化指标的测定
引用本文:孙海涛,耿忠诚,崔波,刘伯铭,吕世玺,任道平.蓝狐GDF-8多态性及其血液生化指标的测定[J].特产研究,2009,31(3):11-13.
作者姓名:孙海涛  耿忠诚  崔波  刘伯铭  吕世玺  任道平
作者单位:黑龙江八一农垦大学动物科技学院,黑龙江大庆163319
摘    要:随机选取50只蓝狐,提取基因组DNA进行聚合酶链式反应(PCR),获得GDF-8基因第3外显子序列,测得BB和AB两个基因型。然后对两种基因型蓝狐碱性磷酸酶(ALP)、血清白蛋白(ALB)、血清总蛋白(TP)进行了测定。结果表明:、水平ALPTP在两基因型个体间差异不显著(P〉0.05),而血清白蛋白水平差异极显著(P〈0.01)。

关 键 词:蓝狐  GDF-8  PCR-SSCP  血液生化指标

Measurement of GDF-8 polymorphism and Blood biochemical parameters in Arctic Foxes
SUN Hai-tao,GENG Zhong-cheng,CUI Bo,LIU Bo-ming,LU Shi-xi,REN Dao-ping.Measurement of GDF-8 polymorphism and Blood biochemical parameters in Arctic Foxes[J].Special Wild Economic Animal and Plant Research,2009,31(3):11-13.
Authors:SUN Hai-tao  GENG Zhong-cheng  CUI Bo  LIU Bo-ming  LU Shi-xi  REN Dao-ping
Institution:(College of Animal Science and Technology of Heilongjiang Bayi Agricultural University, Daqing, 163319 China )
Abstract:The third exon of GDF- 8 was amplified by PCR from genomic DNA of 50 arctic foxes (Alopex lagopus).Two genotypes, BB or AB, was detected. Afterwards three blood biochemical parameters were measured in different genotypes of arctic foxes. The results showed that the differences in ALP and TP levels between two genotypes were not significant (P 〉0.05); however, the differences in ALB between them were significant (P 〈0.01).
Keywords:GDF-8  PCR-SSCP
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