| 3，5-二硝基水杨酸法测定番茄废渣中糖分含量的研究 |
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| 引用本文： | 巩 莉,华 颖,刘大群,陈文烜. 3，5-二硝基水杨酸法测定番茄废渣中糖分含量的研究[J]. 保鲜与加工, 2014, 14(3): 37-42 |
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| 作者姓名： | 巩 莉 华 颖 刘大群 陈文烜 |
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| 作者单位： | 浙江省农业科学院食品科学研究所，浙江省果蔬保鲜与加工技术研究重点实验室,安徽农业大学茶与食品科技学院;浙江省农业科学院食品科学研究所，浙江省果蔬保鲜与加工技术研究重点实验室;浙江省农业科学院食品科学研究所，浙江省果蔬保鲜与加工技术研究重点实验室;浙江省农业科学院食品科学研究所，浙江省果蔬保鲜与加工技术研究重点实验室 |
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| 基金项目： | 国家公益性行业（农业）科研专项（201303079） |
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| 摘 要： | 番茄废渣经微生物发酵可制成蛋白饲料。本试验以番茄废渣为原料,研究3,5-二硝基水杨酸（DNS）法测定番茄废渣及其发酵产物中还原糖及总糖含量的最优方案。结果表明,测定波长为520 nm,DNS试剂用量为1.5 mL,显色时间为5 min,盐酸浓度为6 mol/L,控制在20 min内完成测定。此方法测得的结果精密度高、重现性好,平均回收率可达99.85%,RSD为0.671%,表明采用DNS法测定番茄废渣中的糖分含量准确、可行。
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| 关 键 词： | 番茄废渣 总糖 还原糖 DNS法 |
Study on Determination of Sugar Content from Tomato Residue with 3, 5-Dinitrosalicylic Acid Colorimetry |
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| Affiliation: | GONG Li, HUA Ying, LIU Da-qun, CHEN Wen-xuan(1.Food Science Institute, Zhejiang Academy of Agricultural Science,Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Hangzhou 310021, China; 2. College of Tea with Food Science and Technology, Anhui Agricultural University, Hefei 230031, China) |
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| Abstract: | Tomato residue could be made into protein feed through microbial fermentation. The method of DNS was applied in this article in order to analyze the content of reducing sugar and total sugar in tomato residue and its fermentation product. The result showed that, the optimal conditions as follows, wave lengh was 520 nm, the volume of DNS solution was 1.5 mL, reaction time was 5 minutes, concentration of HCl was 6 mol/L and ensuring complete determination within 20 minutes. The results of the experiment had the characteristics of high precision, good repeatability, the average recovery rate was 99.85%, and RSD was 0.671%. It was showed that using DNS method to determine the sugar content of tomato residue was accurate and feasible. |
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| Keywords: | tomato sla total sugar reducing sugar DNS method |
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