七彩山鸡新城疫病毒的分离鉴定与免疫保护试验

用SPF鸡胚从疑似七彩山鸡新城疫病鸡群中分离到一株病毒，进行HA及HI试验以及病毒中试验。结果表明，血凝作用可被NDV阳性血清所抑制，而不能被AIV（H9亚型）、EDS76所抑制，经ND标准阳性血清处理的病料接种的鸡胚未见死亡，证明分离毒为NDV。动物回归试验表明，分离毒肌肉注射非免疫七彩山鸡能使之发病、死亡，出现与自然病例一致的症状与病变，但肌注SPF鸡只感染，不出现临床症状。其生物学特性NHAT为快、HOT为8min、ICPI为1．73、IVPI为1．45、MDT为44h。表明该七彩山鸡NDV分离株与鸡F48E9株在生物学特性上表现出不同的特性。用分离毒株制成油乳剂灭活疫苗，分别以分离毒株和Lasota株的灭活苗免疫广西麻鸡和七彩山鸡，进行疫苗免疫效果比较和交叉免疫保护试验。结果表明，分离毒株灭活苗免疫组鸡各个时期所产生的HI抗体效价和Lasota灭活苗免疫纽鸡的抗体效价相差不明显，各组的升降规律也基本一致；Lasota灭活苗免疫七彩山鸡对分离毒株的攻击只能80％保护。而其他各免疫组的无论是对分离毒株还是F48E9株的攻击均能100％保护。说明NDV不同毒株之间的毒力和抗原性存在一定的差异。

Isolation Identification and Protective Immunity Test of Newcastle Disease Virus from Pheasant

By using SPF chicken embryo,a strain of disease virus was isolated from suspected pheasants Newcastle Disease.The results of the HA and HI tests and HIV test showed that the Hemagglutination could be inhibited by NDV positive serum,but not AIV(H9 subtypes) and EDS76.There was no death of inoculation chicken embryo treated by ND standard positive serum,which proved isolated virus was NDV.Animal regression test showed that the isolated virus injected through muscular could enable non-immune pheasant the incid...