一个水稻生物产量突变体的遗传分析

突变体是研究基因功能的重要材料。除自然突变和物理、化学方法外，T—DNA插入诱变、转座子插入诱变等分子生物学手段也得到广泛的应用。而水稻全基因组测序的完成和水稻分子标记技术发展，使得通过图位克隆法从非插入突变的水稻突变体获得相关基因变得相对容易，目前，在水稻上通过图位克隆已克隆到一些重要的基因。该研究在转Bar基因水稻后代T1代群体中发展了一个高生物产量突变体，并对该突变体进行遗传分析，为该基因的克隆和功能分析提供参考。

Genetic Analysis of a Biomass Mutant in Oryza sativa

Objective] The study aimed to reveal the genetic model of a biomass mutant in Oryza sativa. Method] ln the process of seresning and iden-tification of Bar-transgenic rice, a biomass mutant was found in 10 lines of TI progenies. The mutant was investigated for genetic analysis and agronomic traits by herbicide spraying and PCR amplification. Result] The segregation ratio is consistent with mendelian law(3:1). The mutant assumed not only higher plant height, wider straw and earlier florescence, but also more tillers, bigger spikes and resuhantly higher biomass. PCR detections indicated that no co-segregation was observed between mutant traits and target gene(Bar) in the T-DNA inserted, proving that the mutant is not caused by the insertion of T-DNA containing target gene (Bar). Conclusion] Our study may avail to understand the cloning of mutant gene and the mechanism of the mutant gene on biomass.