| 几个地方绵羊品种线粒体DNA(mtDNA)细胞色素b基因多态性研究 |
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| 引用本文： | 巩元芳,李祥龙,刘铮铸,吴建华,张艳英. 几个地方绵羊品种线粒体DNA(mtDNA)细胞色素b基因多态性研究[J]. 中国兽医学报, 2006, 26(2): 213-215 |
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| 作者姓名： | 巩元芳 李祥龙 刘铮铸 吴建华 张艳英 |
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| 作者单位： | 1. 河北科技师范学院,动物科学系,河北,昌黎,066600
2. 河北科技师范学院,动物科学系,河北,昌黎,066600;河北农业大学,动物科技学院,河北,保定,071001 |
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| 摘 要： | 用12种限制性内切酶分析了6个地方绵羊品种线粒体DNA（mtDNA）细胞色素b（cytb）基因的RFLP。结果表明，在71只绵羊个体中，检出的14种限制性态型可归结成4种单倍型，其间的差异主要来源于几个限制性位点的点突变；4种单倍型间和6个绵羊群体间的平均遗传距离（D）分别为0．412％和0．041％，遗传多态程度（π）为0．028％，说明遗传多样性较为贫乏；单倍型聚类结果和群体聚类结果提示，我国地方绵羊品种的起源可能是单一的。
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| 关 键 词： | 地方绵羊品种 线粒体DNA 细胞色素b基因 限制性内切酶 |
| 文章编号： | 1005-4545(2006)02-0213-03 |
| 收稿时间： | 2004-06-29 |
| 修稿时间： | 2004-06-29 |
mtDNA Cytochrome B Gene Polymorphisms on Some Chinese Indigenous Sheep Breeds |
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| GONG Yuan-fang,LI Xiang-long,LIU Zheng-zhu,WU Jian-hua,ZHANG Yan-ying. mtDNA Cytochrome B Gene Polymorphisms on Some Chinese Indigenous Sheep Breeds[J]. Chinese Journal of Veterinary Science, 2006, 26(2): 213-215 |
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| Authors: | GONG Yuan-fang LI Xiang-long LIU Zheng-zhu WU Jian-hua ZHANG Yan-ying |
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| Affiliation: | 1. Department of Animal Science, Hebei Normal University of Science and Technology, Changli, Hebei 066600,China;2. College of Animal Science, Hebei Agricultural University, Baoding, Hebei 071001, China |
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| Abstract: | A total of 12 restriction endonucleases were used to analyse in mtDNA of the cytochrome b(Cytb) gene within 6 indigenous sheep breeds.Among the 71 animals,15 restriction morphs were detected,which could be sorted into 4 haplotypes.Differences existed between haplotypes came from several dot mutations.The average genetic distance among 4 haplotypes and among 6 populations was 0.412% and 0.041% respectively and the value of average sequence variation was 0.028%.The results suggested that the genetic diversity within the indigenous sheep breeds was very scarce.It could be inferred that Chinese indigenous sheep originated from one maternal ancestor according to the molecular dendrogram among haplotypes and populations. |
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| Keywords: | PCR-RFLP |
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