金康卡百合离体培养和愈伤诱导的初步研究

目的]百合组培快繁技术途径.方法]以金康卡百合的子房、花柱、花丝为外植体,研究不同灭菌时间、不同培养基对外植体组织培养过程的影响.结果]在愈伤诱导培养基上培养两周左右外植体出现膨大,培养20 d左右,即有淡绿色愈伤组织出现;不同的NAA和BA浓度对愈伤诱导影响较大,激素浓度为0.5 mg/L NAA和1.5 mg/L BA的MS培养基对促进子房膨大和愈伤诱导效果最佳.结论]不同的灭菌时间对外植体污染率的影响不同,均随着灭菌时间的延长呈显著下降的趋势.金康卡百合的子房、花柱、花丝形成愈伤组织能力有一定差异,用子房作外植体比花柱、花丝更易产生愈伤组织且存活率较高.

Preliminary Study on in Vitro Culture and Callus-induced of Ornamental Lily

Objective and Method]With the ovary, style, filament of Jinkangka lily as the explant,the effects of different sterilizing time and mediums on the explant tissue culture process were studied. Result]Light green callus tissue appear when the explants is cultrured for about 20 d.That is , different concentrations of NAA and BA have great impact on the callus induction. Hormone concentration of 0.5 mg/L NAA and 1.5 mg/L BA MS medium have the best impact on promoting the ovary swelling and callus induction formation.Conclusion]The results showed that the effects of different sterilizing time on the contamination rate of the explant were different and they all showed downtrend with the prolonging of the sterilizing time.There are certain differences in the formation of callus ability among the Jinkangka lily ovary, style and filament. It is easier to form callus and have higher survival rates when we use the ovary as explants than use style and filament tissue. The explants were enlarged when it is cultured on the callus induced culture medium for about two weeks.