| 水牛子宫内膜腺上皮细胞和基质细胞的分离·培养和生物学特性研究(英文) |
| |
| 引用本文： | 庄新杰,段亚苹,黄怡,宁淑芳,张明.水牛子宫内膜腺上皮细胞和基质细胞的分离·培养和生物学特性研究(英文)[J].Agricultural Science&Technology,2009(3). |
| |
| 作者姓名： | 庄新杰 段亚苹 黄怡 宁淑芳 张明 |
| |
| 作者单位： | 广西大学动物繁殖研究所;广西亚热带生物资源保护利用重点实验室; |
| |
| 基金项目： | Supported by the Innovation Foundation For Postgraduate of Guangxi University(2008105930905D001) ;;the Tackle Key Program in Science and Technology of Science and Technology Bureau of Guangxi Province(0815008-2-4)~~ |
| |
| 摘 要： | Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying biological mechanism of embryo implantation and uterine diseases. Method] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique were used to isolate BESC and BEGEC, then immunocytochemical method and TRYPAN-Blue assay were used to determine the purity and survival rate of isolated cells. Result] The BESC and BEGEC were successfully isolated and cultured while immunocytochemical method and cell count method demonstrated that the purity was over 90%. The result of TRYPAN-Blue assay shown that survival rate of BESC and BEGEC was 91% and 78% respectively. Conclusion] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique could isolate BESC and BEGEC with high purity.
|
| 关 键 词： | 性细胞 子宫内膜基质细胞 水牛 分离 腺上皮细胞 培养箱 细胞角蛋白 细胞接种 混合酶 培养皿 |
Isolation, Cuture and Biological Characteristic Analysis of Stromal and Glandular Epithelial Cells of Buffalo (Bubalus bubalis) Endometrium |
| |
| ZHUANG Xin-jie,DUAN Ya-ping,HUANG Yi,NING Shu-fang,ZHANG Ming Animal Reproduction Institute,Guangxi University,Guangxi Key Laboratory of Subtropical Bioresource Conservation , Utilization,Nanning.Isolation, Cuture and Biological Characteristic Analysis of Stromal and Glandular Epithelial Cells of Buffalo (Bubalus bubalis) Endometrium[J].农业科学与技术(英文版),2009(3). |
| |
| Authors: | ZHUANG Xin-jie DUAN Ya-ping HUANG Yi NING Shu-fang ZHANG Ming Animal Reproduction Institute Guangxi University Guangxi Key Laboratory of Subtropical Bioresource Conservation Utilization Nanning |
| |
| Institution: | ZHUANG Xin-jie,DUAN Ya-ping,HUANG Yi,NING Shu-fang,ZHANG Ming Animal Reproduction Institute,Guangxi University,Guangxi Key Laboratory of Subtropical Bioresource Conservation , Utilization,Nanning 530005 |
| |
| Abstract: | Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying biological mechanism of embryo implantation and uterine diseases. Method] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique were used to isolate BESC and BEGEC, then immunocytochemical method and TRYPAN-Blue assay were used to determin... |
| |
| Keywords: | Buffalo Endometrial epithelial cell stromal cell Immunocytochemistry |
| 本文献已被 CNKI 等数据库收录！ |
