| 牛副流感病毒3型RT—PCR检测方法的建立 |
| |
| 引用本文： | 刘晓乐,张敏敏,陈颖钰,胡长敏,陈焕春,郭爱珍.牛副流感病毒3型RT—PCR检测方法的建立[J].中国奶牛,2011(22):1-4. |
| |
| 作者姓名： | 刘晓乐 张敏敏 陈颖钰 胡长敏 陈焕春 郭爱珍 |
| |
| 作者单位： | 1. 华中农业大学农业微生物学国家重点实验室,武汉,430070
2. 华中农业大学动物科技学院,武汉,430070 |
| |
| 基金项目： | 基金项目:农业公益性行业科研专项(201003060;2008030181:现代农业(肉牛)产业技术体系专项经费资助 |
| |
| 摘 要： | 参照GenBank中公布的牛副流感病毒3型（Bovineparainfluenzavirus3，BPIV-3）全基因序列，针对BPIV-3特异性NP蛋白保守基因设计一对引物，建立了BPIV-3的RT—PCR诊断方法。其最佳扩增退火温度为58．1℃，引物浓度为1．0μmol／L。采用该方法扩增BPIV-3参考病毒．能扩增出425bp预期大小的特异性片段，而扩增牛病毒性腹泻／黏膜病病毒、牛传染性鼻气管炎病毒、牛合胞体病毒、猪瘟病毒以及牛支原体、大肠埃希氏菌、牛巴氏杆菌和沙门氏菌等常见病毒和细菌均呈阴性结果。对参考病毒进行梯度稀释检测，结果证明该法检测BPIV-3的灵敏度可达10^-3FCID50／0．1mL。
|
| 关 键 词： | PCR 检测方法 牛副流感病毒3型 |
Establishment of RT-PCR Method to Detect Bovine Parainfluenza Virus 3 |
| |
| LIU Xiao-le,ZHANG Min-min,CHEN Ying-yu,HU Chang-min,CHEN Huan-chun,GUO Ai-zhen.Establishment of RT-PCR Method to Detect Bovine Parainfluenza Virus 3[J].China Dairy Cattle,2011(22):1-4. |
| |
| Authors: | LIU Xiao-le ZHANG Min-min CHEN Ying-yu HU Chang-min CHEN Huan-chun GUO Ai-zhen |
| |
| Institution: | LIU Xiao-le1,2,ZHANG Min-min1,CHEN Ying-yu3,HU Chang-min1,CHEN Huan-chun1,GUO Ai-zhen1,2(1.The State Key Laboratory of Agricultural Microbiology,Wuhan 430070,2.College of Veterinary Medicine,3.College of Animal Science,Huazhong Agricultural University,Wuhan 430070) |
| |
| Abstract: | With the primers based on the complete genome sequence of bovine parainfluenza virus 3（BPIV-3） in GenBank, a PCR method for bovine parainfluenza virus 3 detection was developed. The optimum condition for annealing temperature was 58.1℃, primer concentration was 1.0μmol/L. By using this method, the specific fragment of 425 bp was amplified from BPIV-3 template, but not from other common virus and bacteria, such as bovine respiratory syncytial virus, bovine viral diarrhoea virus, infectious bovine rhinotracheitis virus and swine fever virus, mycoplasma bovis strain HB0801, Escherichia coll., Pasteurella muhocida type A and Salmonella typhimurium. The sensitivity of this PCR method was 10-3 TCID50/0.1mL. |
| |
| Keywords: | RT-PCR Detection Bovine parainfluenza virus 3（BPIV-3） |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
