| 用重组的牛巴贝斯虫棒状体蛋白1作为ELISA诊断抗原进行牛群中牛巴贝斯虫病的血清流行病学调查 |
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| 引用本文： | 王慧英,马利青,陆艳,蔡其刚,王戈平,叶成玉.用重组的牛巴贝斯虫棒状体蛋白1作为ELISA诊断抗原进行牛群中牛巴贝斯虫病的血清流行病学调查[J].中国奶牛,2011(22):41-43. |
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| 作者姓名： | 王慧英 马利青 陆艳 蔡其刚 王戈平 叶成玉 |
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| 作者单位： | 1. 青海省湟中县鲁沙尔兽医工作站,湟中,811600
2. 青海省畜牧兽医科学院,西宁,810016 |
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| 基金项目： | 资金项目:国家科技部国际科技合作;序号:2007DFA31350 |
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| 摘 要： | 用重组的牛巴贝斯虫棒状体蛋白1（Bc—RAP-1）作为ELISA诊断抗原，对采自青海省湟中县的120份奶牛血清样品，进行抗Bc—RAP-1特异性抗体的检测。结果检出7份阳性，阳性率为5．83％，说明该地区牛群存在牛巴贝斯虫的感染。
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| 关 键 词： | 牛巴贝斯虫 棒状体蛋白1 ELISA 牛 |
Seroepidemiological Investigate on Bovine babesiosis with ELISA by Recombinant Rhoptry- Associated Protein 1 of Babesia bovis as Diagnosis Antigen |
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| WANG Hui-ying,MA Li-qing,LU Yan,CAI Qi-gang,WANG Ge-ping,YE Cheng-yu.Seroepidemiological Investigate on Bovine babesiosis with ELISA by Recombinant Rhoptry- Associated Protein 1 of Babesia bovis as Diagnosis Antigen[J].China Dairy Cattle,2011(22):41-43. |
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| Authors: | WANG Hui-ying MA Li-qing LU Yan CAI Qi-gang WANG Ge-ping YE Cheng-yu |
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| Institution: | WANG Hui-ying1,MA Li-qing2,LU Yan2,CAI Qi-gang2,WANG Ge-ping2,YE Cheng-yu2(1.Veterinary Station of Lushaer Town in Huangzhong County,Qinghai 811600,2.Qinghai Academy of Animal and Veterinary Science,Xining 810016) |
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| Abstract: | The Recombinant purified Rhoptry-Associated Protein 1 Antigen of Babesia bovis （Bc-RAP-1）was tested in an enzyme- linked immunosorbent assay （ELISA）for detection against Bc-RAP-1 antibodies in cows from Huangzhong county of Qinghai province. Field serum samples were collected from one farm were examined for the diagnosis of Babesiaosis infection by using the ELISA. Of the 120 samples from cows analyzed, 7 （5.83%）samples from cows were positive for antibodies to Babesia bovis. These results suggested that Babesia boris were infected in cows from Huangzhong county of Qinghai province. |
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| Keywords: | Babesia bovis Rhoptry-Associated Protein 1 ELISA Cows |
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