九节茶种质遗传多样性ISSR分析

为进一步研究九节茶的遗传特性，为育种工作提供理论指导，利用ISSR分子标记试验方法研究了37个九节茶种源的遗传多样性，从100条引物中筛选出17条引物，分别利用POPGENE 1.32软件及NTSYS软件对九节茶种源进行了基于ISSR的遗传多样性分析及种源聚类分析。结果表明：17条引物共扩增出105个位点，其中多态性位点87个，多态性位点百分率65.71%；37个种源间遗传多样性较低，福建、广东、江西、浙江和广西5个种群之间的遗传多样性为0.1770，种内遗传多样性为0.0990，基因分化系数为0.4405，即种群间遗传变异占种群遗传变异的44.05%，55.95%的遗传变异在种内进行。由种群间的遗传分化系数计算的基因流Nm=0.6350。遗传相似系数为0.7048~0.9143，平均0.8096。聚类分析表明，在遗传相似系数为0.81处，37个种源可以分为5类，聚类结果并不能完全反映出种源的地理分布，但可以部分反映地理分布的特点，为种质资源的鉴定打下良好基础。

The Research on Genetic Diversity Analysis by ISSR Markers of Sarcandra glabra (Thunb.) Nakai

In order to further study the genetic characteristics of Sarcandra glabra （Thunb.） Nakai,ISSR markers were involved to analyze genetic diversity in 37 accessions of Sarcandra glabra （Thunb.） Nakai germplasms.Among the 100 ISSR molecular markers,there were 17 primers showing good diversity and clear bands.The genetic diversity analysis and cluster analysis were based on the amplification results with POPGENE 1.32 and NTSYS software.It was found that the 105 sites were found by 17 primers,and 87 sites showed polymorphism,the percentage of polymorphic loci was 65.71%,the genetic diversity of the tested 5 populations in the Fujian,Guangdong,Jiangxi,Zhejiang and Guangxi,was 0.1770,and the intra species genetic diversity was 0.0990.The coefficient of gene differentiation （Gst） was 0.4405,and the gene flow Nm was 0.6350.It was indicated that 44.05% population variation came from the interspecific variation,and the others came from intraspecific variation.The variance range of genetic similarity coefficient was 0.7048-0.9143,with an average of 0.8096.Cluster analysis showed that 37 kinds of species could be clustered into 5 groups at a genetic distance coefficient of 0.81.The cluster result indicated that the genetic distance between each small population was related to the environment in which different population of Sarcandra glabra （Thunb.） Nakai grew.However,the genetic distance had no correlation with geographic distance.