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头孢氨苄的紫外分光光度法测定
引用本文:王学立,程亮亮,温俊柳,汪雪雁,薛秀恒,祁克宗. 头孢氨苄的紫外分光光度法测定[J]. 中国奶牛, 2011, 0(18): 59-61
作者姓名:王学立  程亮亮  温俊柳  汪雪雁  薛秀恒  祁克宗
作者单位:安徽农业大学茶与食品科技学院,合肥,230036
摘    要:本研究应用紫外分光光度计法测定了头孢氨苄注射液中头孢氨苄的含量,以建立一种准确、简单的测定方法,用于头孢氨苄注射液的含量测定。头孢氨苄在紫外光区有最大吸收值,且最大吸收波长为261nm。以261nm为测定波长做标准曲线,得到回归方程为:y=0.0224x,R2=0.9996。此法快捷简便,适用于兽药及动物性食品中药物残留分析。

关 键 词:紫外分光光度计  头孢氨苄注射液  快速检测

Determination of Cefalexin Injection by Ultraviolet Spectrophotometric Method
WANG Xue-li,CHENG Liang-liang,WEN Jun-liu,WANG Xue-yan,XUE Xiu-heng,QI Ke-zong. Determination of Cefalexin Injection by Ultraviolet Spectrophotometric Method[J]. China Dairy Cattle, 2011, 0(18): 59-61
Authors:WANG Xue-li  CHENG Liang-liang  WEN Jun-liu  WANG Xue-yan  XUE Xiu-heng  QI Ke-zong
Affiliation:WANG Xue-li,CHENG Liang-liang,WEN Jun-liu,WANG Xue-yan,XUE Xiu-heng,QI Ke-zong(College of Tea and Food Science and Technology,Anhui Agricultural University,Hefei 23006)
Abstract:In order to establish an accurate and simple method for the content of cephalexin injection determination, a method by UV spectrophotometer was conducted in this study. Results showed that the maximum wavelength of cephalexin in ultraviolet was 261nm. The standard curve was established and the regression equation was y=0.0224x, R2=0.9996. This method is fast, simple and applicable to analysis veterinary drugs and drugs residues in animal food.
Keywords:UV spectrophotometer  Cephalexin injection  Rapid detection
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