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Introgression of a major gene for high grain protein content in some Indian bread wheat cultivars
Authors:J. Kumar  V. Jaiswal  A. KumarN. Kumar  R.R. Mir  S. KumarR. Dhariwal  S. TyagiM. Khandelwal  K.V. PrabhuR. Prasad  H.S. BalyanP.K. Gupta
Affiliation:a Molecular Biology Laboratory, Department of Genetics & Plant Breeding, Ch. Charan Singh University, Meerut 250004, India
b Division of Genetics, Indian Agriculture Research Institute, New Delhi 110012, India
c Division of Crop Improvement, Indian Institute of Pulses Research, Kanpur 208024, India
d International Crop Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502324, Greater Hyderabad, India
e Indian Agricultural Statistics Research Institute, Pusa Campus, New Delhi 110012, India
Abstract:In bread wheat, high grain protein content (GPC) determines nutritional value, processing properties and quality of the end-product. In view of this, marker-assisted selection (MAS) was performed for introgression of a major gene for high GPC (Gpc-B1) into 10 wheat genotypes. As a result, 124 BC3F5/F6 progenies with Gpc-B1 were developed and evaluated in multi-location field trials. Significant interaction of Gpc-B1 with the recipient parent genotypes and the environment was noticed. However, a total of seven MAS-derived progenies with significantly higher GPC (14.83-17.85%) than their recipient parental genotypes and having no yield penalty were obtained. In these selected progenies, no significant negative correlation of grain yield with GPC (%) or protein yield was observed suggesting that GPC could be improved without yield penalty. This study thus suggested that MAS in combination with phenotypic selection is a useful strategy for development of wheat genotypes with high GPC associated with no loss in yield.
Keywords:Bread wheat   Grain protein content   Leaf rust   MAS
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