A set of near-isogenic lines for blast resistance genes with an Indica-type rainfed lowland elite rice (Oryza sativa L.) genetic background |
| |
Authors: | Yohei Koide Leodegario A Ebron Hiroshi KatoHiroshi Tsunematsu Mary Jeanie Telebanco-YanoriaNobuya Kobayashi Masao YokooSachio Maruyama Tokio ImbeYoshimichi Fukuta |
| |
Institution: | a Japan International Research Center for Agricultural Sciences, 1-1, Ohwashi, Tsukuba, Ibaraki 325-8686, Japan b International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines c National Institute of Crop Science, 2-1-18, Kannondai, Tsukuba, Ibaraki 305-8517, Japan d Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Ibaraki 305-8572, Japan e National Agricultural Research Center for the Kyushu Okinawa Region, Suya, Goushi, Kumamoto 861-1192, Japan |
| |
Abstract: | A set of near-isogenic lines for blast resistance genes was developed by using an Indica-type elite rice variety, IR49830-7-1-2-2, suitable for the rainfed lowland conditions in the tropics, as a genetic background. Initially, we revealed that IR49830-7-1-2-2 harbors five blast resistance genes - Pia, Pib, Pik-s, Pita, and Pi11(t) - by using a differential system involving 19 selected standard blast isolates from the Philippines. Based on this result, we developed nine near-isogenic lines (NILs) targeting eight resistance genes - Pik, Pi7(t), Pi3, Pi5, Pita-2, Piz-5, Pish, and Pi9 - by recurrent backcrossing. The introgression of each resistance gene in the NILs was confirmed by reaction patterns to the blast isolates, allelism tests, and DNA marker analysis. In addition, a genome-wide DNA marker survey revealed that most of the chromosome regions in each NIL were of the IR49830-7-1-2-2 type. The agricultural characteristics of most of the developed NILs were almost the same as those of IR49830-7-1-2-2. Moreover, with one exception, they showed submergence tolerance similar to IR49830-7-1-2-2. The developed NILs could be used as a multiline variety suitable for the rainfed lowland in the tropics. |
| |
Keywords: | Rice (Oryza sativa L ) Blast (Pyricularia grisea (Cooke) Sacc ) Resistance gene Near-isogenic line Multiline variety |
本文献已被 ScienceDirect 等数据库收录! |
|