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| 绵羊产单核细胞李斯特菌的分离及鉴定 | |
| 引用本文： | 张杰,李辉,孙晓林,骆学农,郑亚东,伏小平. 绵羊产单核细胞李斯特菌的分离及鉴定[J]. 甘肃农业大学学报, 2007, 42(2): 22-25 |
| 作者姓名： | 张杰 李辉 孙晓林 骆学农 郑亚东 伏小平 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070;中国农业科学院兰州兽医研究所动物病原生物学国家重点试验室甘肃省动物寄生虫病重点试验室,甘肃,兰州,730046;山东省科学院能源研究所,山东,济南,250014 2. 中国农业科学院兰州兽医研究所动物病原生物学国家重点试验室甘肃省动物寄生虫病重点试验室,甘肃,兰州,730046 3. 甘肃农业大学动物医学院,甘肃,兰州,730070;中国农业科学院兰州兽医研究所动物病原生物学国家重点试验室甘肃省动物寄生虫病重点试验室,甘肃,兰州,730046 4. 甘肃农业大学动物医学院,甘肃,兰州,730070 |
| 基金项目： | 中国农业科学院所长基金 |
| 摘 要： | 采用生化反应、溶血试验、致病力试验等常规方法对从疑似产单核细胞李斯特菌(Listeria monocytogenes,LM)患病羊脑组织分离的病原作了初步鉴定.通过PCR扩增hlyA基因部分保守序列,测序证实其与已报道的核苷酸序列的同源性达到100%,从而确诊该菌为产单核细胞李斯特菌. |
| 关 键 词： | 绵羊 产单核细胞李斯特菌 PCR鉴定 |
| 文章编号： | 1003-4315（2007）02-0022-04 |
| 修稿时间： | 2006-06-21 |
Isolation and identification of Listeria monocytogenes in sheep |
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| ZHANG Jie,LI Hui,SUN Xiao-lin,LUO Xue-nong,ZHENG Ya-dong,FU Xiao-ping. Isolation and identification of Listeria monocytogenes in sheep[J]. Journal of Gansu Agricultural University, 2007, 42(2): 22-25 | |
| Authors: | ZHANG Jie LI Hui SUN Xiao-lin LUO Xue-nong ZHENG Ya-dong FU Xiao-ping |
| Affiliation: | 1. College of Veterinary Medicine, Gansu Agricultural University,Lanzhou 730070, China; 2. Key Laboratory of Veterinary Parasitology of Gansu Province,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,CAAS,Eanzhou 730046,China; 3. Energy Research Institute of Shandong Academy of Sciences,Jinan 250014,China |
| Abstract: | Listeria monocytogenes(LM),as the important member of the genus Listeria,is a zoonotic and food-borne pathogen,and the disease is of significance in public health.In this study,the pathogen separated from sheep brain issues possibly infected by LM was primarily identified using biochemical and virulence and other conventional indentification methods.Parts of conserved nucleotide fragments of hlyA gene was amplified by PCR,and in view of the complete homology in nucleotide sequence of the hlyA gene in this study and that previously reported in the GenBank,the pathogen was finally determined as LM.The study provided an important reference method for specifically diagnosing and detecting LM. |
| Keywords: | sheep Listeria monocytogenes PCR identification |
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