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| 腐蹄病节瘤拟杆菌纤毛蛋白与绵羊白细胞介素-2融合基因表达载体的构建及表达 | |
| 引用本文： | 王克坚,Om Dhungyel,余兴龙,冯书章,赵宝华,J R Egerton,朱平,殷震. 腐蹄病节瘤拟杆菌纤毛蛋白与绵羊白细胞介素-2融合基因表达载体的构建及表达[J]. 中国兽医学报, 2002, 22(2): 131-133 |
| 作者姓名： | 王克坚 Om Dhungyel 余兴龙 冯书章 赵宝华 J R Egerton 朱平 殷震 |
| 作者单位： | 1. 中国农业科学院吉林特产研究所,吉林,吉林市,132109 2. 澳大利亚悉尼大学 3. 解放军军需大学军事兽医研究所,吉林,长春,130062 |
| 基金项目： | 国家“九五”科技攻关项目 ( 96 -0 0 5 -0 2 -0 3-0 9)，国家科技部科研院所技术开发研究专项资金项目 |
| 摘 要： | |
| 关 键 词： | 腐蹄病 节瘤拟杆菌 纤毛蛋白基因 绵羊白细胞介素2基因 |
| 文章编号： | 1005-4545(2002)02-0131-03 |
| 修稿时间： | 2000-10-06 |
Construction of a Plasmid pmPili IL-2 Expressing a Fusion Construct of D.no dosus Pili Gene and Ovine IL-2 Gene |
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| Om Dhungyel,J R Egerton. Construction of a Plasmid pmPili IL-2 Expressing a Fusion Construct of D.no dosus Pili Gene and Ovine IL-2 Gene[J]. Chinese Journal of Veterinary Science, 2002, 22(2): 131-133 | |
| Authors: | Om Dhungyel J R Egerton |
| Abstract: | A fusion expression plasmid was constructed by inserting the ovine IL-2 gene into PME290-Pili in the Pili gene at downstream 619 bp.The plasmid harbouring ovine IL-2 sequence was designated pmPili IL-2.The fusion protein was expressed by transforming the pmPili IL-2 into the competent host cell BL-21(DE3).Specific reaction between PiliIL-2 fusion protein and the antiserum of D.nodosus serotype E Pili was observed by cross electrophoresis.The molecular weight of the recombinant fusion protein was about 33 000 by SDS-PAGE and analyzed with thin layer.The recombinant Pili IL-2 fusion gene was expressed at higher level with about 5.49% of the total proteins of the transformant host cell BL-21(DE3). |
| Keywords: | footrot D.nodosus Pili gene OviIL-2 gene |
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