| 木糖筛选剂在辣椒遗传转化上的应用 |
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| 引用本文： | 王秀峰,王学国,马艺荞,王健鹂,张悦.木糖筛选剂在辣椒遗传转化上的应用[J].辣椒杂志,2014(1):30-32. |
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| 作者姓名： | 王秀峰 王学国 马艺荞 王健鹂 张悦 |
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| 作者单位： | 吉林省蔬菜花卉科学研究院,长春130033 |
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| 基金项目： | 国家863计划“出口型优质抗病甜椒杂交育种技术研究与应用”（子专题）（2002AA207013-8） |
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| 摘 要： | 为了缓解转基因食品安全性问题,利用安全的木糖筛选系统,开展了辣椒遗传转化技术研究;设置不同的木糖和蔗糖浓度组合,探讨木糖选择压力,初步确立20/5,25/5(g/L,木糖/蔗糖)两个组合为最适木糖筛选浓度;利用农杆菌介导法将pCAMBIA3301-xylose导入辣椒,开展了辣椒安全标记遗传转化技术研究。
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| 关 键 词： | 木糖筛选系统 木糖异构酶 辣椒 遗传转化 |
Xylose as Selective Agent in Hot Pepper Genetic Transformation |
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| Wang Xiufeng Wang Xueguo Ma Yiqiao Wang Jianli Zhang Yue.Xylose as Selective Agent in Hot Pepper Genetic Transformation[J].Journal of China Capsicum,2014(1):30-32. |
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| Authors: | Wang Xiufeng Wang Xueguo Ma Yiqiao Wang Jianli Zhang Yue |
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| Institution: | Wang Xiufeng Wang Xueguo Ma Yiqiao Wang Jianli Zhang Yue ( Jilin Academy of Vegetable and Flower Sciences, Changchun 130033, China ) |
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| Abstract: | To alleviate the genetically modified food safety condition, xylose screening system has been used to study the genetic transformation of hot pepper; two combinations such as 20/5 and 25/5 （g/L, xylose/sucrose）have been initially established as the optimal coneentration of xylose screening; and the pCAMBIA3301-xylose vector has been transfered into hot pepper with the method of agrobacterium tumefaciens in this study. |
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| Keywords: | xylose screening system xylose isomerase hot pepper （Capsicum annuum L ） genetic transformation |
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