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菊苣组织培养及无性系建立的研究
引用本文:高琳娜,陈鹏彦,姜长阳. 菊苣组织培养及无性系建立的研究[J]. 内蒙古农业科技, 2008, 0(3): 54-56
作者姓名:高琳娜  陈鹏彦  姜长阳
作者单位:辽宁师范大学生命科学学院,辽宁,大连,116029
摘    要:以具有有利变异性状的菊苣叶柄为材料,进行了组织培养及无性系建立的研究。结果证明:MS+BA0.4mg/L+2,4-D丁酯1.6mg/L是诱导叶柄形成具有分化能力愈伤组织的理想培养基:1/2MS+AgNO3 20.5mg/L+BA0.3mg/L+NAA0.1mg/L是愈伤组织和不定芽分化的理想培养基;1/3MS+IAA0.2~0.4mg/L是变异菊苣不定芽生根的理想培养基;炉灰渣是菊苣试管苗移栽的理想基质。

关 键 词:菊苣  无性系  快速繁殖

In Vitro Culture of Chicory and its Clones Establishment
Affiliation:GAO Lin-na (College of Life Science, Liaoning Normal University, Dalian 116029,China)
Abstract:A study on the technique of tissue culture and establishment of asexual system of Cichorium intybus was conducted. The results indicated that the ideal medium for induction and differentiation of buds from stem segments are MS+BA0.4mg/L+ 2,4-D1.6mg/L and 1/2 MS+ AgNO3 20.5mg/L+BA0.3mg/L+NAA0.1mg/L, respectively, The medium of 1/3MS+IAA0.2-0.4 mg/L is suitable for root growth.
Keywords:Cichorium intybus  Clone,Rapid propagation
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