Effects of pyriproxyfen, a juvenile hormone analog, on development of the mealworm, Tenebrio molitor |
| |
Authors: | N Aribi S Lakbar N Soltani |
| |
Institution: | a Laboratoire de Biologie Animale Appliquée, Département de Biologie, Université Badji Mokhtar, 23000 Annaba, Algeria b Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium |
| |
Abstract: | Pyriproxyfen, an analog of the juvenile hormone (JH) in insects, has been evaluated in vivo and in vitro on an important stored product pest, the mealworm Tenebrio molitor. On newly ecdysed pupae, this insect growth regulator (IGR) was applied topically (at 0.10 and 0.20 μg/insect). Enzyme immunoassay measurements showed a decrease of the ecdysteroid titer in the hemolymph of treated specimens. In addition, the treatment induced an increase of the protein concentration in the hemolymph, however, there was no significant effect on the protein band pattern. Using in vitro pupal integument explant cultures, pyriproxyfen (at 1 and 10 μM in the medium) caused a significant inhibition of ecdysteroid amounts and of the thickness of new cuticle secreted. These new results are discussed in relation to the physiology of this IGR with a JH action. |
| |
Keywords: | Pyriproxyfen Juvenile hormone analog Ecdysteroid titer Cuticle thickness Hemolymph proteins Development |
本文献已被 ScienceDirect 等数据库收录! |
|