Target site insensitivity and mutational analysis of acetylcholinesterase from a carbofuran-resistant population of Colorado potato beetle, Leptinotarsa decemlineata (Say) |
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Authors: | Hyo Jeong Kim Kyong Sup Yoon |
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Affiliation: | a Molecular and Cellular Biology Program, University of Massachusetts, Amherst, MA 01003, USA b Department of Entomology, University of Massachusetts, Amherst, MA 01003, USA c School of Public Health and Health Sciences, University of Massachusetts, Amherst, MA 01003, USA d Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA |
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Abstract: | The BERTS strain of Colorado potato beetle (CPB) was found to be highly resistant to N-methyl carbofuran but relatively susceptible to azinphosmethyl. N-Methyl carbofuran resistance was found to correlate well with acetylcholinesterase (AChE) insensitivity. In becoming resistant to N-methyl carbofuran, the AChE of the BERTS strain became more sensitive to N-propyl carbofuran inhibition. This negative cross-insensitivity correlated well to the increased relative toxicity of the BERTS strain to N-propyl carbofuran compared to the susceptible SS strain. BERTS beetles were sorted into BERTS-R and BERTS-S substrains using their AChE activity profiles. Sequence comparisons of AChE cDNAs from the two substrains revealed the presence of the point mutation that results in the S291G substitution previously found in the AChE of the azinphosmethyl-resistant AZ-R strain of CPB. A novel mutation present only in BERTS-S CPB, however, resulted in an additional I392T substitution in the AChE and apparently reverses the resistance conferring properties of the S291G substitution. |
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Keywords: | Colorado potato beetle Carbofuran resistance AChE insensitivity Point mutations Negative cross-resistance Negative cross-insensitivity |
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