| 红三叶组织培养及再生植株 |
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| 引用本文： | 孟玉玲,韩榕.红三叶组织培养及再生植株[J].草业学报,1994,3(2):51-54. |
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| 作者姓名： | 孟玉玲 韩榕 |
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| 作者单位： | 兰州大学生物系，山西师范大学生物系 |
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| 摘 要： | 本文采用ＭＳ，Ｂ５和ＰＣ一Ｌ２作为基本培养基，在不同激素组合条件下对红三叶（Ｔｒｉｆｏｌｉｕｍｐｒａｔｅｎｓｅ）子叶及下胚轴进行组织培养。在ＭＳ，Ｂ５和ＰＣ一Ｌ２附加２ｍｇ／Ｌ２，４－Ｄ和０．５ｍｇ／ＬＢＡ的培养基上，子叶及下胚轴愈伤组织诱导率均在８９％以上。愈伤组织继代在Ｂ５无机盐十三倍ＭＳ有机成分＋３％蔗糖＋０．５ｍｇ／Ｌ２，４－Ｄ＋０．５ｍｇ／Ｌ６ＢＡ０．２ｍｇ／ＬＮＡＡ，＋１０ｍｇ／ＬＶｃ的培养基上生长良好。愈伤组织在Ｂ５附加０．１ｍｇ／Ｌ６ＢＡ＋０．２ｍｇ／ＬＩＢＡ的培养基上再生植株。下胚轴在ＭＳ附加２ｍｇ／Ｌ６ＢＡ＋１ｍｇ／ＬＮＡＡ的培养基上直接分化形成丛生苗，转入无激素ＮＳ培养基上再生根。实验表明ＶＥ，Ｖｃ，ＰＶＰ和活性碳对愈伤组织褐化有抑制作用，１０ｍｇ／ＬＶｃ抗褐化作用最好。
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| 关 键 词： | 红三叶 组织培养 再生植株 |
Tissue Culture in vitro and Plant Regeneration from Callus as well as Explant of Red Clover(Trifolium pratense L.) |
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| Meng Yuling, Hao Yunpeng,Jia Jingfen.Tissue Culture in vitro and Plant Regeneration from Callus as well as Explant of Red Clover(Trifolium pratense L.)[J].Acta Prataculturae Sinica,1994,3(2):51-54. |
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| Authors: | Meng Yuling Hao Yunpeng Jia Jingfen |
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| Abstract: | |
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| Keywords: | Red Clover Tissue culture Plant regeneration Inhibition to browning reaction |
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