斑马鱼和鲢鱼对微囊藻毒素与孔雀石绿的行为反应

鱼类行为能准确地反映环境变化，并且随着污染物浓度的变化而发生改变，因此将毒素敏感鱼类的行为作为标记，用以监测环境是可行的。本研究用人工饲养，对环境毒素敏感的斑马鱼和对环境毒素有较强吸收能力的鲢鱼作为指示生物，检测其行为对水体中微囊藻毒素(MC)和孔雀石绿(MG)的反应。结果表明，斑马鱼和鲢鱼在2 µg/L MC浸泡下未出现显著的行为反应，而在5 µg/L的MC浸泡下，斑马鱼摆尾频率在10 min 后，移动速度在40 min后显著性下降；鲢鱼摆尾频率与移动速度均在40 min后显著性下降。另一方面，0.5 mg/L MG处理下，斑马鱼未出现显著性行为变化，而鲢鱼摆尾频率与移动速度均在35 min后显著性下降。

Behavior response of zebrafish, Danio rerio and silver carp, Hypophthalmichthys molitrix to Microcystins and Malachite green

Behavior index of fish accurately reflected the alteration of environment. In addition, it could monitor the existence of contamination and followed the concentration change of contamination, so it was feasible to use the sensitive fish to monitor the environment. This study used Danio rerio, which was a sensitive fish to environmental toxin and Hypophthalmichthysmolitrix, which had strong absorbability to environmental toxin to be the eucoen, and behavior index of these two fishes was used to monitor the existence of microcystins (MC) and malachite green (MG). The results showed that, when exposed to 2 µg/L MC-LR, no significant behavior response of Danio rerio and Hypophthalmichthys molitrix were observed. When exposed to 5 µg/L MC-LR, tail beat frequency of Danio rerio and Hypophthalmichthys molitrix decreased significantly after 10 min and 40 min, respectively. Swimming velocity of Danio rerio and Hypophthalmichthys molitrix both decreased significantly after 40 min. When exposed to 0.5 mg/L MG, no significant behavior response of Danio rerio was observed, whereas tail beat frequency and swimming velocity of Hypophthalmichthys molitrix decreased significantly after 35 min.