Characterization,inheritance, and molecular study of opaque leaf mutant in mungbean (<Emphasis Type="Italic">Vigna radiata</Emphasis> (L.) Wilczek) |
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Authors: | Ornuma Rungnoi Sonthichai Chanprem Theerayut Toojinda Ian Godwin Chris Lambrides Peerasak Srinives |
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Institution: | 1.Center for Agricultural Biotechnology,Kasetsart University,Kamphaeng Saen, Nakhon Pathom,Thailand;2.Department of Plant Production Technology,King Mongkut’s Institute of Technology Ladkrabang,Bangkok,Thailand;3.Department of Agronomy,Kasetsart University,Kamphaeng Saen, Nakhon Pathom,Thailand;4.Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology,Kasetsart University,Kamphaeng Saen, Nakhon Pathom,Thailand;5.School of Land and Food Science,University of Queensland,St Lucia, Brisbane,Australia |
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Abstract: | Chlorophyll-deficiency mutants are useful as genetic markers and as materials for studying the photosynthesis process. We
characterized the inheritance of the gene controlling an opaque leaf (OL) trait in mungbean (Vigna radiata (L.) Wilczek). An F2 population was developed from the cross between the OL mutant and ‘Berken’, an Australian mungbean cultivar, to study inheritance
and molecular tagging. The population was advanced by selfing to produce F5 lines from which two normal lines and two OL lines were randomly chosen to study chlorophyll content, seed growth and development,
and seed cell morphology. The chlorophyll content in opaque leaf was lower than normal and thus was expected to have lower
photosynthetic activity. This resulted in yellowish and shrinking pods and seeds within 15 days after flowering, while those
from normal plants extended their growth up to 18 days. The cotyledon transfer cells of the OL plants deformed at 12 days
and deteriorated at 14 days after flowering. The OL trait was controlled by a single recessive ol gene which was independent from the genes controlling petiole color and growth habit. We used 193 AFLP primer combinations
to tag this gene and found that the marker AGG/ATA was linked with the ol gene at a distance of 3.4 cM. |
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