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荧光法测定南瓜子萌芽期色氨酸含量的变化
引用本文:崔竹梅,顾霞,黄有如,朱益波.荧光法测定南瓜子萌芽期色氨酸含量的变化[J].种子,2009,28(11).
作者姓名:崔竹梅  顾霞  黄有如  朱益波
作者单位:常熟理工学院生物与食品工程系,江苏,常熟,215500
摘    要:研究了南瓜子的萌发条件和萌发过程中游离色氨酸含量的变化.结果显示,对南瓜种子进行45~55℃水煮30min,再用纯净水浸泡4~6 h处理,再置于湿润的滤纸上发芽效果较好.种子萌芽48 h,种苗中游离色氨酸含量最高,达到5.11 mg/g(WW),之后呈下降趋势.

关 键 词:南瓜子  萌芽  荧光分光光度法  色氨酸

The Change of Tryptophan Content in Pumpkin Seeds during Germination Determined by Fluorescence Spectrophotometry
CUI Zhu-mei,GU Xia,HUANG You-ru,ZHU Yi-bo.The Change of Tryptophan Content in Pumpkin Seeds during Germination Determined by Fluorescence Spectrophotometry[J].Seed,2009,28(11).
Authors:CUI Zhu-mei  GU Xia  HUANG You-ru  ZHU Yi-bo
Abstract:The condition of germination and the change of tryptophan content in pumpkin seeds during germination were studied.The results showed that soaking in water(45-55℃)for 30 hours before being laid on the wet filter papers could promote the germination percentage of the pumpkin seeds.The content of tryptophan at the second 24 hours in pumpkin seeds was the highest,which was up to 5.09 mg/g(WW),and then decreased.
Keywords:pumpkin seed  germination  fluorescence spectrophotometry  tryptophan
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