| 棉花黄萎病菌毒素的分离提纯及致萎活性测定 |
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| 引用本文： | 甘 莉,吕金殿. 棉花黄萎病菌毒素的分离提纯及致萎活性测定[J]. 西北农林科技大学学报(自然科学版), 1991, 19(1): 49-54 |
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| 作者姓名： | 甘 莉 吕金殿 |
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| 作者单位： | 陕西省植物保护研究所(甘莉)，陕西省植物保护研究所(吕金殿) |
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| 摘 要： | 经超滤,Con.A—Scpharosc 4B亲和层析,从棉花黄萎病菌的Czapck′s培养液中,分离纯化致萎毒素。成分分析和致萎活性测定结果表明:棉花黄萎病菌产生的毒素是一种致萎力极强的糖蛋白。其含糖量为14.74％,蛋白质量为85.26％,其致萎活力是半纯化液的10～15倍,活力回收为76％。
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| 关 键 词： | 棉花 黄萎病菌 致萎毒素 糖蛋白 Con.A-Scpharosc 4B亲和层析 |
Isolation and Purification of Cotton Verticillium Wilt Pathogen and the Bioassay of wilting Phytotoxin |
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| Can Li LQ Jindian. Isolation and Purification of Cotton Verticillium Wilt Pathogen and the Bioassay of wilting Phytotoxin[J]. Journal of Northwest A&F University（Natural Science Edition）, 1991, 19(1): 49-54 |
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| Authors: | Can Li LQ Jindian |
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| Affiliation: | Shaanxi frovicial flam Protection Research Institute |
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| Abstract: | The wilting toxin was separated and purified from Czapck's medium liquid of cotton vcrlicillium wilt pathogen by ultrafiltration and Con.A-Scpharosc 4B affinity labeling. The component analysis and the results from bioassay of wilting phytotoxin showed that toxin was a kind of glyco-protein with a strong wilting ability, which contains 14.74% of carbonhydratc and 85.26% of protein .The toxin wilting activity was 12-15 times higher than that of half crude toxin , and the retraction wilting activity was 76%. |
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| Keywords: | cotton wilting toxin verticillum wilting pathogen Glyco-protein Con.A-Sepharose 4B affinity labeling |
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