Enzymes Present in Pancreatic Extracts of the Dolphin Coryphaena hippurus

Pancreatic extract of adult dolphin Coryphaena hippurus was found to have a broad range of proteolytic activity with two peaks, one at pH 5–6 and another at pH 10, when casein was used as a substrate. Enzymes similar in activity to those of pepsin, trypsin, amylase, lipase and collagenase were detected in the extracts by the use of specific chromogenic substrates for each enzyme. The relationship between these enzyme activities, and the formulation of diets for culture of this species, is discussed.