小豆抗锈病诱导剂的筛选

Screening inducers on adzuki bean rust resistance

Four common inducers, salicylic acid (SA), 1-aminocyclopropane-1-carboxylic acid (ACC), benzothiadiazole (BTH) and methyl jasmonate (MeJA), were selected to evaluate the effects of inducers on the resistance of adzuki bean to rust by investigating urediospores germination and the adzuki bean resistance to Uromyces vignae. The results showed that four inducers had no effects on the urediospores germination. However, they can significantly induce adzuki bean resistance to U. vignae. Among these four inducers, 0.25 mg·mL^-1 ACC can significantly reduce the disease index, which was 45.06% lower than the control. Aditionally, seedlings treated with ACC exhibited a typical "triple reaction", which indicated that this induced resistance may relate with the endogenous ethylene biosynthesis in adzuki bean. Then the adzuki bean seedlings were treated with an ethylene receptor inhibitor, 1-methylcyclopropene (1-MCP) alone or combined with ACC. The results showed that 1-MCP alone had no significant effect on the growth and rust resistance of adzuki bean. However, 1-MCP could eliminate the "triple reaction" and did not affect the induced resistance caused by ACC when it used combined with ACC. Results obtained in current study suggested that ACC can significantly induce the resistance of adzuki bean to the rust. Moreover, we speculate that ACC-induced rust resistance of adzuki bean may not depend on ethylene signaling pathway.