尖孢镰刀菌番茄专化型中SNARE蛋白FolSso1的功能分析

 SNARE(Soluble N-ethylmaleimide-sensitive factor attachment protein receptor)蛋白保守存在于丝状真菌中,在膜泡转运的过程中起着关键的作用。番茄枯萎病是由尖孢镰刀菌番茄专化型(Fusarium oxysporum f. sp. lycopersici,Fol)引起的,严重威胁着番茄的生产。我们使用反向遗传学的方法来研究番茄枯萎病菌中SNARE蛋白FolSso1的功能,实验结果发现FolSSO1的基因缺失突变体菌丝生长速率降低,且产孢数量减少。另外,FolSSO1基因的缺失导致突变体相较于野生型菌株对细胞壁压力与细胞膜压力更加敏感。然后,在番茄果实和番茄植株的致病性实验中,我们发现FolSSO1的缺失并没有引起Fol致病性显著的变化。综上所述,本研究发现FolSSO1可以调控Fol营养生长,繁殖和对环境压力的响应过程,然而对Fol的致病过程并没有显著的调控作用。

Functional analysis of the SNARE protein FolSso1 in Fusarium oxysporum f. sp. lycopersici

SNARE (Soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins are conserved in filamentous fungi and play key roles in the process of membrane transport. Tomato vascular wilt disease is caused by Fusarium oxysporum f. sp. lycopersici (Fol), which is a serious threat to tomato production. We used reverse genetics to study the function of FolSsol protein, which is a SNARE protein in Fol. The results showed that the gene deletion mutants of FolSSO1 reduced the mycelial growth rate and decreased the number of sporulation. In addition, the deletion mutants of FolSSO1 were more susceptible to cell wall pressure and cell membrane stress than the wild-type strain. In addition, we found that the deletion of FolSSO1 has no effects on the virulence of Fol through pathogenicity experiments of tomato plant and tomato fruits. In summary, this study found that FolSSO1 can regulate vegetative growth, reproduction and response to environmental stress, but there is no significant role of FolSso1 involved in the pathogenicity of Fol.