一株PVYNTN-NW黑龙江马铃薯分离物的检测鉴定

 马铃薯Y病毒(Potato virus Y,PVY)是马铃薯、烟草等茄科作物上的重要病毒,在与寄主共同进化过程中产生了许多株系。本文从黑龙江马铃薯样品中得到PVY分离物A12。ELISA结果表明A12被PVY^O的单克隆抗体特异性识别。A12开放阅读框为9 186个核苷酸,编码3 061个氨基酸,与SYR-II-Be1分离物的核苷酸和氨基酸序列一致率均最高,分别为98.3%和99.2%。系统发育分析发现A12与PVY^NTN-NW株系SYR-II型的分离物聚类到一起;重组分析表明,A12是N-605和O_z的重组体,重组类型与SYR-II-Be1相同。综合以上结果表明,A12属于PVY^NTN-NW株系SYR-II型。但与常见PVY^NTN-NW株系分离物在珊西烟引起叶脉坏死不同,A12产生花叶症状。A12辅助成分-蛋白酶在182位和245位的氨基酸均为精氨酸,而其它PVY^NTN-NW株系分离物为赖氨酸。本研究结果可为黑龙江马铃薯PVY的早期检测和有效防控提供理论指导。

Detection and identification of a Potato virus Y (PVY) NTN-NW isolate from potato in Heilongjiang,China

Potato virus Y(PVY)is an economically important virus which causes destructive losses to the production of solanaceous crops including potato and tobacco. During the co-evolution with host, PVY isolates have evolved into many different strains. In this study, PVY isolate A12 was obtained from potato leaves in Heilongjiang, China. ELISA results indicated that A12 was specifically recognized by the monoclonal antibody specific for PVY^N. The open reading frame of A12 was 9 186 nt, encoding a polyprotein of 3 061 amino acids, and shared the highest nucleotide and amino acid identities of 98.3% and 99.2% with isolate SYR-II-Be1. Phylogenetic analysis showed that A12 was clustered with isolates belonging to SYR-II type of PVY^NTN-NW. Recombinant analysis indicated that A12 was recombinant of isolates N-605 and O_z and shared same recombination pattern with isolate SYR-II-Be1. Taken together, all the results described above indicated that A12 belonged to the PVY^NTN-NW strain (SYR-II type). However, A12 induced symptom of mosaic, instead of veinal necrosis, in Nicotiana tabacum cv. Xanthi. The amino acids at positions 182 and 245 in helper component-proteinase of A12 were both Arginine, instead of Lysine in other PVY^NTN-NW isolates. The results of this study provide theoretical guide for the early detection and effective control of PVY in potato from Heilongjiang.