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| 云南滇西高原粳稻区白叶枯病菌遗传多样性初探 | |
| 引用本文： | 梁斌,王敬文,明风,戴陆园,叶鸣明,沈大棱.云南滇西高原粳稻区白叶枯病菌遗传多样性初探[J].上海交通大学学报(农业科学版),2003,21(Z1):38-42. |
| 作者姓名： | 梁斌 王敬文 明风 戴陆园 叶鸣明 沈大棱 |
| 作者单位： | 1. 复旦大学,生命科学院遗传所,遗传工程国家重点试验室,上海,200433;云南省农业科学院,品种资源站,昆明,650205 2. 复旦大学,生命科学院遗传所,遗传工程国家重点试验室,上海,200433 3. 云南省农业科学院,品种资源站,昆明,650205 |
| 基金项目： | 教育部生物多样性与区域生态安全重点学科建设项目，云南省自然科学基金重点项目资助(200C0010Z) |
| 摘 要： | 用IS-PCR和rep-PCR法分析了17个采自云南滇西高原粳稻区的水稻白叶枯病菌(Xanthomonasoryzaepv.O-ryzae)和7个其他地区的水稻白叶枯病菌的群体遗传多样性。用2个适合中国水稻白叶枯病菌的引物J3和ERIC对这24个菌系基因组DNA进行PCR扩增反应,J3引物有14条条带,ERIC引物有13条条带,2个引物呈现18种谱型。用PHYLIPVersion3.6软件UPGMA聚类分析法分析白叶枯病菌系之间的遗传距离,可将24个病原菌分为4簇,3个来自菲律宾的白叶枯病菌与中国的菌系距离较远,云南高原粳稻区分离的菌系十分复杂,4个簇中都有,但主要集中在2个簇中,共有14个菌系。 |
| 关 键 词： | 高原粳稻区 白叶枯病菌 遗传多样性 |
| 文章编号： | 1671-9964(2003)S0-0038-05 |
| 修稿时间： | 2003年3月31日 |
Genetic Diversity of Xanthomonas oryzae pv. Oryzae from the West of Yunnan Japonica Rice Planted Area |
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| Institute of Genetics,State Key Laboratory of Genetic Engineering,School of Life Sciences,Fudan Univ.,Shanghai.Genetic Diversity of Xanthomonas oryzae pv. Oryzae from the West of Yunnan Japonica Rice Planted Area[J].Journal of Shanghai Jiaotong University (Agricultural Science),2003,21(Z1):38-42. | |
| Authors: | Institute of Genetics State Key Laboratory of Genetic Engineering School of Life Sciences Fudan Univ Shanghai |
| Institution: | Institute of Genetics,State Key Laboratory of Genetic Engineering,School of Life Sciences,Fudan Univ.,Shanghai 200433, 2. Institute of Crop Genetic Resources,Yunnan Academy of Agriculture Sciences,Kunming 650205,China) |
| Abstract: | IS PCR and rep PCR were used to analyze the genetic diversity in a population of 17 strains of Xanthomonas oryzae pv. Oryzae collected from the west of Yunnan japonica rice planted area and 7 strains from other areas. Genomic DNA from 24 strains were amplified with 2 specific primers J3 and ERIC by PCR and revealed 18 haplotypes, 14 bands by J3 primer and 13 bands by ERIC primer. Dendrograms were generated from the data sets obtained by using PHYLIP Version 3.6 UPGMA analysis. 21 strains from China were grouped in 4 clusters, 3 strains from the Philippines was out of 4 clusters. Among 17 strains from the west of Yunnan, 14 strains mainly grouped in 2 clusters. |
| Keywords: | japonica rice planted area Xanthomonas oryzae pv Oryzae genetic diversity |
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