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| 红皮云杉同工酶位点及酶系统选择 | |
| 引用本文： | 张含国,杨大海,王风君,徐桂生,罗玉亮.红皮云杉同工酶位点及酶系统选择[J].东北林业大学学报,2000,28(2):9-12. |
| 作者姓名： | 张含国 杨大海 王风君 徐桂生 罗玉亮 |
| 作者单位： | 1. 黑龙江省林业科学研究所,哈尔滨,150040 2. 黑龙江省伊春林管局 3. 黑龙江省兴隆林业局 4. 大兴安岭集团公司林木种子站 |
| 基金项目： | 黑龙江省重点课题及国家“九五”科技攻关项目 |
| 摘 要： | 通过对３种研磨提取液、５种凝脱及电泳缓冲系统以及１４种等位酶系统的筛选。结果表明，红皮云杉同工酶种子研磨提取液采用ｃ、凝胶及电泳缓冲系统采用＃８、＃１１较好。＃８胶可染ＡＡＴ、ＡＤＨ、ＧＤＨ、ＤＩＡ、＃１１胶可染ＭＤＨ、ＭＥ、ＩＤＨ、６ＰＧＤ、ＰＧＩ、ＡＣＯ、１６个位点可作为红皮云杉遗传标记。 |
| 关 键 词： | 红皮云杉 遗传标记 同位酶位点 酶系统选择 |
Selection of Allozyme Loci and Enzyme Systems of Picea koraiensis |
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| Zhang Hanguo,Yang Dahai,Wang Fengjun,Xu Guisheng,Luo Yuliang.Selection of Allozyme Loci and Enzyme Systems of Picea koraiensis[J].Journal of Northeast Forestry University,2000,28(2):9-12. | |
| Authors: | Zhang Hanguo Yang Dahai Wang Fengjun Xu Guisheng Luo Yuliang |
| Abstract: | The seeds of PI'cea koraiensis from 11 sites throughout the northeast area in China were tested with 3 kinds of enzyme extraction bufTers. 5 kinds of gel electrophoresis buffers and 14 kinds of enzyme system. The results indicated that c extraction buffer and #8. #l I gel electrophoresis buffer systems were used better in the seeds of P. koraiensis than others. #8 gel electrophoresis was used to stain well in AAT, GDH and DIA. while #if in MDH, ME, IDH. 6PGD* PGI and ACO. 16 allozyme loci are taken as genetic marker for P. koraiensis. |
| Keywords: | Picea koraiensis Allozyme Genetic marker |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |