Identification of RAPD markers linked to linolenic acid genes in rapessed |
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Authors: | Christelle Jourdren Pierre Barret Raymonde Horvais Régine Delourme Michel Renard |
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Affiliation: | (1) Station d'Amélioration des Plantes, INRA, BP 29, F-35650 Le Rheu, France |
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Abstract: | Summary The inheritance of the low linolenic acid content (derivated from mutant lines) in rapeseed was investigated. Molecular techniques of gene mapping through RAPD markers were applied on a microspore-derived progeny from a high × low linolenic acid F1 hybrid. Bulked segregant analysis made it possible to test rapidly number of RAPD primers. Two linkage groups of 6 markers (72.7 cM and 75.6 cM) were determined. Each corresponded to a major QTL which explained 24% and 30.7% of the total phenotypic variation of the linolenic acid content. It was confirmed that two independant mutations were implied in the low linonenic acid content. |
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Keywords: | doubled haploid gene mapping genetic control linolenic acid mutant plant breeding RAPD rape seed Brassica napus |
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