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Identification of RAPD markers linked to linolenic acid genes in rapessed
Authors:Christelle Jourdren  Pierre Barret  Raymonde Horvais  Régine Delourme  Michel Renard
Institution:(1) Station d'Amélioration des Plantes, INRA, BP 29, F-35650 Le Rheu, France
Abstract:Summary The inheritance of the low linolenic acid content (derivated from mutant lines) in rapeseed was investigated. Molecular techniques of gene mapping through RAPD markers were applied on a microspore-derived progeny from a high × low linolenic acid F1 hybrid. lsquoBulked segregant analysisrsquo made it possible to test rapidly number of RAPD primers. Two linkage groups of 6 markers (72.7 cM and 75.6 cM) were determined. Each corresponded to a major QTL which explained 24% and 30.7% of the total phenotypic variation of the linolenic acid content. It was confirmed that two independant mutations were implied in the low linonenic acid content.
Keywords:doubled haploid  gene mapping  genetic control  linolenic acid  mutant  plant breeding  RAPD  rape seed  Brassica napus
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