细毛羊羔羊超数排卵及体外胚胎生产移植

【目的】研究羔羊日龄、羔羊体重、超排方法、精卵共孵育时间对细毛羔羊超排效果及卵母细胞体外受精效果的影响。【方法】用国产FSH对4～9周龄羔羊进行两种超排方法处理,回收可用卵母细胞1 032枚,体外受精后移植受体98只,产羔25只。【结果】不同周龄羔羊只均获卵数和可用卵数之间差异显著(P<0.05);不同激素方案处理组的发育卵泡两种处理方案在发育卵泡数上无显著差异(P>0.05),但在回收率和可用卵数上差异显著(P<0.05);不同体重组只均发育卵泡数和可用卵数之间差异显著(P<0.05)。【结论】4～6周龄的细毛羊羔羊超排获得卵母细胞数目较多;重复超排的羔羊超排效果不理想;同时,将受精液pH值调为7.5～7.6,并将精卵共孵育时间控制在22～26 h,卵母细胞体外受精及发育效果较好。

Fine Wool Sheep Superovulation and in vitro Lamb Embryos Production Transplantation

【Objective】The experiment was conducted to study the in vitro fertilization effect of lamb age,lamb weight,superovulation method sperm-oocyte co-incubation time on fine wool lamb superovulation and oocyte.【Method】 Domestic 4-9 weeks FSH lambs were chosen to go through two superovulation methods processing.1 032 oocytes available were recovered,and after in vitro fertilization and transplantation receptors were 98,lambing 25.【Result】The number of retrieved oocytes and oocyte number available of different weeks old lamb are greatly different(P<0.05);the number of different hormone treatment group growth follicle two treatment options in the development showed no significant difference(P>0.05),but the recovery rate and the number of oocytes available were significantly different(P<0.05);there was a big difference(P<0.05) between the growth follicle number and oocyte number available.【Conclusion】The results showed that 4 to 6 weeks of age of fine-wool sheep lamb superovulated oocytes obtained quite a lot of numbers,the number of repeated superovulation and the sheep superovulation effect is not ideal;at the same time,the semen pH value affected was adjusted to be between 7.5 and 7.6,and the sperm-oocyte co-incubation time control was within 22-26 h.Oocytes in vitro fertilization and development effect was good.