响应面法优化超声波辅助提取玛咖总黄酮的工艺研究

目的]优化玛咖总黄酮的超声波辅助提取工艺.方法]采用Box - Behnken试验设计及响应面分析法对玛咖总黄酮的超声波提取工艺进行优化,建立回归模型.结果]得到回归方程:Y=2.080 +0.074 X1 -0.099X2-0.057X3-0.697X12 -0.209X22 -0.336X32 +0.005X1X2+0.05X1X3+0.169X2X3；最佳提取工艺为乙醇浓度70.3;、料液比1∶27、超声时间28.4 min,此工艺条件下玛咖总黄酮的提取率为2.113;,与模型预测值吻合.结论]响应面回归方程与试验结果拟合性好,可用于实际预测,为玛咖总黄酮的提取提供了参考.

Optimization of Ultrasonic-assisted Extraction of Flavonoids from Maca by Response Surface Methodology

【Objective】In order to optimize the ultrasonic-assisted extraction condition of flavonoids from Maca.【Method】Box-Behnken design and response surface analysis were adopted to establish regression model of extraction of flavonoids from Maca.【Result】Regression equation was Y=2.080 +0.074X1-0.099 X2-0.057X3-0.697X12-0.209X22-0.336X32 +0.005X1X2 +0.05X1X3 +0.169X2X3.The optimal extraction conditions were ethanol concentration of 70.3%,solid-to-liquid of 1∶ 27 and ultrasonic time of 28.4 min.Under the conditions,the yield of flavonoids was 2.113%,and it was well fitted the experimental data of model.【Conclusion】The developed regression equation can be well fitted with experimental results and used for actual prediction,and it also provided reference for the extraction of flavonoids from Maca.