盐胁迫下小黑麦碳酸酐酶基因表达及其酶活性分析

目的]探究盐胁迫下小黑麦中碳酸酐酶(CA)基因表达特点及酶活性变化规律.方法]采用0.15M NaCI对两个六倍体小黑麦品种幼苗进行胁迫处理,通过实时荧光定量PCR对胁迫条件下CA基因表达量的变化进行检测,并利用pH计法对相应材料中的碳酸酐酶活性进行测定.结果]盐胁迫条件下两个小黑麦品种幼苗中的CA基因表达量及酶活性均较对照有所增强.随胁迫时间的不同,参试的两个小黑麦品种中CA基因表达量及酶活性变化的幅度有一定差异.结论]盐胁迫对小黑麦中CA基因的表达量和酶活性均具有促进作用.

Expression of Carbonic Anhydrase Gene and Enzyme Activity in Triticale(X Triticosecale Wittmack) Under Salt Stress

Objective]To understand the relationship between the expression of carbonic anhydrase gene and its enzyme activity in triticale under salt stress.Method]Two triticale varieties XXHM 3 and XXHM 5 were stressed with 0.15 M NaCl,and the expression of carbonic anhydrase gene was detected with fluorescence real - time quantitative PCR technique,and the activity of carbonic anhydrase was assayed with the pH method.Result]The expression of carbonic anhydrase gene in triticale was enhanced under salt stress,and carbonic anhydrase gene expression was higher in the two triticale variety under salt stress than normal condition.The activity of carbonic anhydrase was higher under salt stress than that in normal condition.The enzyme activity and expression of carbonic anhydrase were affected more obviously in XXHM 3 than in XXHM 5.Conclusion]The expression of carbonic anhydrase gene and activity of carbonic anhydrase in triticale can be enhanced under salt stress.