东方百合OT系列试管鳞茎的诱导与植株再生体系的建立

[目的]通过对东方百合OT系列品种康佳德奥、罗宾娜试管鳞茎的诱导,建立康佳德奥、罗宾娜的植株再生快繁体系,为进一步建立脱毒快繁体系奠定基础.[方法]采用康佳德奥、罗宾娜不同部位的外植体进行试管鳞茎的诱导,比较用不同部位的外植体诱导的难易及快慢程度,筛选适合的诱导激素、增殖激素及生根激素水平.[结果]在百合OT系列选择外植体诱导时,最好用茎尖作外植体进行诱导;由于罗宾娜、康佳德奥所含内源激素水平不同,因而增殖培养基BA最好控制在1.0～0.5 mg/L;生根激素水平罗宾娜为NAA 0.5mg/L,康佳德奥为NAA0.3 mg/L,生根率为100;,试管苗结鳞茎率大于50;;试管苗移栽在条件容允许的情况下,用山土最为适宜,成活率可达到93;以上.[结论]在百合OT系列选择外植体诱导时,最好用茎尖作外植体进行诱导;罗宾娜的增殖培养基为MS+BA0.5 mg/L+ NAA0.5 mg/L、康佳德奥的增殖培养基为MS+BA0.5 mg/L+ NAA0.1 mg/L;生根激素水平罗宾娜为NAA 0.5 mg/L,康佳德奥为NAA 0.3 mg/L,试管苗移栽最好使用山土.

The Inducing of OT Oriental Lily Bulblet and Establishing of Regeneration System

【Objective】 The paper studied establishing regeneration system by inducing bulblet of OT oriental lily varieties of Concad and Robina.It is basis for the further development of virus-free fast propagation system.【Method】 The induction of difficulty of the degree and speed with different parts of the explants were compared,and optimum level of induction hormones,propagation hormones and proliferation rooting hormone were selected so as to establish propagation system of Concad and Robina.【Result】 The result showed that the best material was bulblet tip with bulblet of OT oriental lily Concad and Robina.Because Concad and Robina themselves contain different endogenous hormones,BA of the best concentration in proliferation medium was controlled within 1.0-0.5 mg/L.For rooting hormones,Robina was NAA0.5 mg/L,Concad’s NAA0.3 mg/L,the rooting rate were all 100%.Tube seedlings transplant was conducted better with the hill soil,and the survival rate could reach as high as 93%.【Conclusion】The best material was bulblet tip with bulblet of OT oriental lily Concad and Robina.The Robina’s proliferation culture medium was MS+BA0.5 mg/L+ NAA0.5 mg/L,Concad’s was MS+BA0.5 mg/L+ NAA0.1 mg/L.Robina’s rooting hormone was NAA0.5 mg/L,Concad’s was NAA0.3 mg/L.The best soil was mountain soil for transplanting plantlets.