Function and anatomy of plant siRNA pools derived from hairpin transgenes |
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Authors: | Bess L Chau Kevin AW Lee |
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Institution: | (1) Department of Botany, University of Hong Kong, Hong Kong, S.A.R., China;(2) Department of Biology, Hong Kong University of Science & Technology, Hong Kong, S.A.R., China |
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Abstract: | Background RNA interference results in specific gene silencing by small-interfering RNAs (siRNAs). Synthetic siRNAs provide a powerful
tool for manipulating gene expression but high cost suggests that novel siRNA production methods are desirable. Strong evolutionary
conservation of siRNA structure suggested that siRNAs will retain cross-species function and that transgenic plants expressing
heterologous siRNAs might serve as useful siRNA bioreactors. Here we report a detailed evaluation of the above proposition
and present evidence regarding structural features of siRNAs extracted from plants. |
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Keywords: | |
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