葡萄籽多酚对免疫抑制小鼠肠道菌群的影响

以葡萄籽多酚为研究对象,通过环磷酰胺诱导建立免疫抑制小鼠模型,利用Illumina MiSeq高通量测序平台探究葡萄籽多酚对免疫抑制小鼠肠道菌群的影响.结果 表明:葡萄籽多酚干预能够提高小鼠免疫器官指数及免疫球蛋白(sIgA、IgM、IgG)的含量;低剂量葡萄籽多酚干预能够提高肠道菌群多样性,促进乳酸杆菌属、拟杆菌属、狄氏副拟杆菌属、双歧杆菌属、苏黎世杆菌属、坦纳拟普雷沃菌属、别样棒菌属、罗姆布茨菌属细菌的生长,抑制巴恩斯氏菌属、埃希氏菌-志贺氏菌属、艾克曼菌属等细菌的生长;同时,乳酸杆菌属及双歧杆菌属等益生菌的增殖,有利于促进短链脂肪酸的产生,进而增强机体免疫能力.

Effect of Grape Seed Polyphenols on Gut Microbiota in Immunosuppressive Mice

With the grape seed polyphenols as research object, an immunosuppressive mice model was established through cyclophospamide (CPA) induction, and Illumina MiSeq high-throughput sequencing platform was used to explore the effect of grape seed polyphenols on the gut microbiota in immunosuppressive mice. The results showed that the grape seed polyphenols intervention could increase the immune organ index and the content of immunoglobulin(sIgA, IgM, IgG) in mice. Furthermore, the low does grape seed polyphenols could increase the diversity of gut microbiota, and promote the growth of Lactobacillus, Bacteroides, Parabacteroides distasonis, Bifidobacterium, Turicibacter, Alloprevotella tannerae, Allobaculum and Romboutsia, and inhibit the growth of Barnesiella, Escherichia/Shigella, Akkermansia. Meanwhile, the proliferation of probiotics such as Lactobacillus and Bifidobacterium was conducive to promoting the production of short-chain fatty acids, thus enhancing the immune ability of the body.