不同脂肪源对卵形鲳鲹肠道微生物菌群的影响

为确定脂肪源种类对卵形鲳鲹肠道微生物结构和组成的影响,本研究通过高通量测序技术分析不同脂肪源对卵形鲳鲹肠道微生物菌群结构、多样性和差异性的影响。以鱼油、磷虾油、豆油、玉米油,1∶1鱼油-豆油、1∶1鱼油-玉米油、1∶1磷虾油-豆油和1∶1磷虾油-玉米油为主要脂肪源,配制了8种等氮等能的饲料投喂640尾卵形鲳鲹。测序结果显示,在卵形鲳鲹肠道内共获得1087662个序列,运算分类单元稀释曲线趋于平缓表明测序深度已经基本覆盖到样品中所有的物种。在门水平上,主要注释到拟杆菌门、厚壁菌门、变形菌门和软壁菌门4个门,且变形菌门为优势菌群。不同脂肪源对ACE指数、Chao1指数、香农-威纳指数和辛普森多样性指数具有显著影响,磷虾油组和1∶1鱼油-豆油组具有较高的肠道微生物丰富度和多样性。主坐标分析和主成分分析表明,不同脂肪源对肠道菌群结构影响显著,这可能与脂肪酸种类和含量有关。本试验结果显示,不同脂肪源对卵形鲳鲹肠道微生物菌群具有显著影响。

Effects of Different Lipid Sources on Intestinal Microbial Flora in Golden Pompano Trachinotus ovatus

High-throughput sequencing method was used to analyze structure,diversity and differences of intestinal bacteria community in golden pompano Trachinotus ovatus with body weight of(82.85±2.36)g fed satiation to eight isonitrogenous and isoenergetic diets with different lipid sources fish oil(FO),krill oil(KO),soybean oil(SO),and corn oil(CO)as ratio of 1∶1 FO-SO,1∶1 FO-CO,1∶1 KO-SO and 1∶1 KO-CO at water temperature of 27-31℃for 8 weeks to evaluate effects of different lipid sources on intestinal microbial flora in golden pompano.A total of 1087662 sequences were obtained,and all species in the sample were substantially covered by OTU rarefaction curve.Bacteroidetes,Firmicutes,Proteobacteria and Tenericutes were mainly detected,with Proteobacteria as the predominant phylum in the intestine of golden pompano.ACE,Chao1,Shannon and Simposon were significantly affected by different lipid sources,and higher richness and diversity of intestinal bacteria in the diets containing KO and 1∶1 FO-SO.Principal co-ordinates analysis(PCoA)and principal component analysis(PCA)revealed that different lipid sources significantly affected intestinal microbiota structure in golden pompano,which was involved in different types and contents of fatty acids.